Jensen C G, Brown S, Pedersen S
Department of Molecular Cell Biology, University of Copenhagen, Denmark.
J Bacteriol. 1994 May;176(9):2502-6. doi: 10.1128/jb.176.9.2502-2506.1994.
We examined the synthesis of individual proteins following depletion of 4.5S RNA by using a strain deficient in the induction of heat shock proteins. We found that initially the synthesis of all proteins was equally affected, and the peptide elongation rate was reduced by approximately 10%. For up to 1 generation time after the onset of inhibition of total protein synthesis, the processing of secreted proteins was unaffected. After further depletion of 4.5S RNA, accumulation of precursors of secreted proteins was observed under some growth conditions.
我们使用一株缺乏热休克蛋白诱导能力的菌株，研究了4.5S RNA耗竭后单个蛋白质的合成情况。我们发现，最初所有蛋白质的合成均受到同等程度的影响，肽链延伸速率降低了约10%。在总蛋白质合成受到抑制开始后的长达1个世代时间内，分泌蛋白的加工过程未受影响。在进一步耗竭4.5S RNA后，在某些生长条件下观察到了分泌蛋白前体的积累。
