Panneton W M, Burton H
Neuroscience. 1985 Jul;15(3):779-97. doi: 10.1016/0306-4522(85)90078-8.
The projections from the medullary and spinal dorsal horns to the dorsolateral pons were investigated in the cat utilizing both the retrograde and anterograde transport of a wheat germ agglutinin-horseradish peroxidase complex and the retrograde transport of the fluorescent dyes Fast Blue and Nuclear Yellow. After injections of wheat germ agglutinin-horseradish peroxidase into the area surrounding the brachium conjunctivum, numerous neurons were labeled ipsilaterally near levels of the obex in the paratrigeminal nucleus. Such neurons were located in connected pockets of neuropil located within the spinal trigeminal tract and along its medial edge. Most of the neurons labeled in the dorsal horns after such injections were found in lamina I. Those found in the medullary dorsal horn were mostly ipsilateral to the injection while those in the spinal dorsal horn were found bilaterally. Some labeled neurons were also found in lamina V of both the medullary and spinal dorsal horns bilaterally. When the injection was centered in either the medial parabrachial nucleus or the Kolliker-Fuse nucleus, a greater number of neurons were labeled ipsilaterally in lamina V of the medullary dorsal horn. Since neurons in lamina I of the medullary dorsal horn also project to the medial thalamus, fluorescent dyes were used to determine if the same neuron might project to both targets. Fast Blue was first injected into either the peribrachial area or the medial thalamus. After an appropriate period, Nuclear Yellow was injected into that target not injected first with Fast Blue. The injection of Nuclear Yellow was always placed on the side of the brain opposite to the first injection. Both dyes were transported retrogradely and were found in neurons located in lamina I of the medullary dorsal horn. However, no double-labeled neurons were seen. In general those labeled after injections of the medial thalamus were more superficial than those labeled after injections of the dorsolateral pons. The anterograde transport of wheat germ agglutinin-horseradish peroxidase was used to determine the termination of the projections from neurons in the medulary dorsal horn and the cervical spinal cord to the peribrachial area. After injections into these areas a moderate to sparse labeling of the lateral parabrachial nucleus and the Kolliker-Fuse nucleus was seen. It was mostly ipsilateral in cases with injections of the medullary dorsal horn but was bilateral following injections into the cervical enlargement.(ABSTRACT TRUNCATED AT 400 WORDS)
利用小麦胚凝集素 - 辣根过氧化物酶复合物的逆行和顺行运输以及荧光染料快蓝和核黄的逆行运输，在猫身上研究了延髓和脊髓背角向脑桥背外侧的投射。将小麦胚凝集素 - 辣根过氧化物酶注射到结合臂周围区域后，在三叉旁核内靠近闩平面的同侧标记了许多神经元。这些神经元位于脊髓三叉神经束内及其内侧边缘相连的神经毡小窝中。此类注射后在背角标记的大多数神经元位于I层。在延髓背角发现的那些神经元大多在注射的同侧，而在脊髓背角发现的神经元双侧都有。在延髓和脊髓背角的V层双侧也发现了一些标记神经元。当注射集中在内侧臂旁核或 Kölliker - Fuse 核时，在延髓背角的V层同侧标记了更多神经元。由于延髓背角I层的神经元也投射到内侧丘脑，因此使用荧光染料来确定同一神经元是否可能投射到两个靶点。首先将快蓝注射到臂旁周围区域或内侧丘脑。经过适当时间后，将核黄注射到未首先注射快蓝的那个靶点。核黄的注射总是放在与第一次注射相反的脑侧。两种染料都逆行运输，并在延髓背角I层的神经元中发现。然而，未见到双标记神经元。一般来说，内侧丘脑注射后标记的神经元比脑桥背外侧注射后标记的神经元更表浅。利用小麦胚凝集素 - 辣根过氧化物酶的顺行运输来确定延髓背角和颈脊髓神经元向臂旁周围区域投射的终止部位。向这些区域注射后，可见外侧臂旁核和 Kölliker - Fuse 核有中度至稀疏的标记。延髓背角注射的情况大多是同侧的，但颈膨大注射后是双侧的。（摘要截短至400字）
