Negrini D, Venturoli D, Townsley M I, Reed R K
Istituto di Fisiologia Umana, Università degli Studi di Milano, Italy.
J Appl Physiol (1985). 1994 Feb;76(2):627-33. doi: 10.1152/jappl.1994.76.2.627.
The permselectivity of the parietal pleura was determined in spontaneously breathing anesthetized rabbits and dogs. In rabbits, we injected intrapleurally 5 ml of 1-g/dl albumin solution containing 100 microCi of 131I-labeled albumin plus 100 microCi of either lactate dehydrogenase (LDH) or alpha 2-125I-macroglobulin. Dogs received 100 ml of 1-g/dl albumin solution containing 100 microCi of 131I-albumin plus 100 microCi of alpha 2-125I-macroglobulin. A transpleural pressure gradient was set, lowering the intracapsular pressure to -30 cmH2O. The solvent drag reflection coefficients (sigma f) were calculated as the ratio between tracer concentrations in capsular and pleural liquid collected at 60-180 min. In rabbits sigma f was 0.44 +/- 0.2 (SD) for albumin, 0.84 +/- 0.1 for LDH, and 0.93 +/- 0.05 for alpha 2-macroglobulin. In dogs sigma f was 0.30 +/- 0.19 for albumin and 0.53 +/- 0.15 for alpha 2-macroglobulin. The hydraulic conductivity of the parietal pleura was 2.18 +/- 1.54 microliters.h-1.cmH2O-1.cm-2 in rabbits and 1.22 +/- 1.13 microliters.h-1.cmH2O-1.cm-2 in dogs. The parietal pleura could be modeled by two pore populations with radii of 83-89 and 156-222 A. The permeability coefficient averaged 0.08-0.21 x 10(-6) cm/s for albumin, 0.06-0.09 x 10(-6) cm/s for LDH, and 0.01-0.03 x 10(-6) cm/s for alpha 2-macroglobulin.
在自主呼吸的麻醉兔和犬身上测定了脏层胸膜的通透选择性。在兔身上，我们经胸膜腔内注射5毫升含100微居里¹³¹I标记白蛋白以及100微居里乳酸脱氢酶（LDH）或α₂-¹²⁵I-巨球蛋白的1克/分升白蛋白溶液。犬接受100毫升含100微居里¹³¹I-白蛋白以及100微居里α₂-¹²⁵I-巨球蛋白的1克/分升白蛋白溶液。设置跨胸膜压力梯度，将囊内压力降至-30厘米水柱。溶剂拖曳反射系数（σf）计算为60至180分钟收集的囊液和胸膜液中示踪剂浓度之比。兔白蛋白的σf为0.44±0.2（标准差），LDH为0.84±0.１，α₂-巨球蛋白为0.93±0.05。犬白蛋白的σf为0.30±0.19，α₂-巨球蛋白为0.53±0.15。兔脏层胸膜的水力传导率为每小时每平方厘米每厘米水柱2.18±1.54微升，犬为每小时每平方厘米每厘米水柱1.22±1.13微升。脏层胸膜可由半径为83 - 89埃和156 - 222埃的两种孔群模拟。白蛋白的渗透系数平均为0.08 - 0.21×10⁻⁶厘米/秒，LDH为0.06 - 0.09×10⁻⁶厘米/秒，α₂-巨球蛋白为0.01 - 0.03×10⁻⁶厘米/秒。
