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AluI、DNase I、苄基核酸酶和博来霉素在中国仓鼠卵巢（CHO）细胞中诱导姐妹染色单体交换

Induction of sister-chromatid exchanges by AluI, DNase I, benzon nuclease and bleomycin in Chinese hamster ovary (CHO) cells.

作者信息

Obe G, Schunck C, Johannes C

机构信息

University GH Essen, Department of Genetics, Germany.

出版信息

Mutat Res. 1994 May 1;307(1):315-21. doi: 10.1016/0027-5107(94)90305-0.

DOI:10.1016/0027-5107(94)90305-0
PMID:7513811
Abstract

Various endonucleases (AluI, DNase I, benzon nuclease) and bleomycin induce sister-chromatid exchanges (SCE) in Chinese hamster ovary (CHO) cells. The frequencies of SCE are elevated in cells with chromosome-type aberrations, only slightly elevated in cells with chromatid exchanges, and in the control range in cells without chromosomal aberrations. These data indicate that SCE are produced when DNA breaks induced in G1 are either not repaired or misrepaired.

摘要

多种核酸内切酶（AluI、DNase I、苄基核酸酶）和博来霉素可诱导中国仓鼠卵巢（CHO）细胞发生姐妹染色单体交换（SCE）。在具有染色体型畸变的细胞中，SCE频率升高；在具有染色单体交换的细胞中，SCE频率仅略有升高；而在无染色体畸变的细胞中，SCE频率处于对照范围内。这些数据表明，当G1期诱导产生的DNA断裂未被修复或错配修复时，就会产生SCE。

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