新制癌菌素可诱导中国仓鼠卵巢细胞出现染色体畸变和姐妹染色单体交换。

Neocarzinostatin induces chromosomal aberrations and sister chromatid exchanges in Chinese hamster ovary cells.

作者信息

Schunck C, Obe G

机构信息

Department of Genetics, University GH Essen, Germany.

出版信息

Mutagenesis. 1995 Jan;10(1):37-42. doi: 10.1093/mutage/10.1.37.

DOI:10.1093/mutage/10.1.37

PMID:7739399

Abstract

Chinese hamster ovary (CHO) cells were treated with neocarzinostatin (NCS) and analyzed for chromosomal aberrations and sister chromatid exchanges (SCE). After treatment the cells were recovered for 9, 20, 26 or 30 h. NCS induces chromosomal aberrations and SCE. SCE were much more frequent in cells with chromosome type aberrations at 20 h recovery time than in those with chromatid type aberrations at 9 h recovery time. In second post-treatment cells at 26 or 30 h recovery time NCS induced chromosomal aberrations but only few SCE.

摘要

用新制癌菌素（NCS）处理中国仓鼠卵巢（CHO）细胞，并分析其染色体畸变和姐妹染色单体交换（SCE）情况。处理后，细胞恢复培养9、20、26或30小时。NCS可诱导染色体畸变和SCE。在恢复20小时时，具有染色体型畸变的细胞中SCE比恢复9小时时具有染色单体型畸变的细胞中更为频繁。在恢复26或30小时的第二次处理后的细胞中，NCS诱导了染色体畸变，但仅诱导了少量SCE。