Morii E, Kosaka J, Nomura S, Fukuda Y, Kitamura Y
Department of Pathology, Osaka University School of Medicine, Suita, Japan.
Neurosci Lett. 1994 Jan 31;166(2):168-70. doi: 10.1016/0304-3940(94)90477-4.
We found cells expressing c-kit receptor and its ligand (Sl factor, SLF) in the retina of rats and mice. c-kit messenger RNA (mRNA) was detected in a limited number of cells in the inner nuclear layer of rats and mice by in situ hybridization. The c-kit-expressing cells were assumed to be a subpopulation of the amacrine cells on the basis of their size and distribution pattern. c-kit protein-containing cells were demonstrated in the mouse retina by using ACK2 monoclonal antibody against the extracellular domain of the mouse c-kit receptor. The c-kit protein was detected in cell bodies and processes of the presumed amacrine cells. Hybridization signals for SLF mRNA were observed in the retinal ganglion cells. The results suggest that the c-kit receptor and its ligand may play some roles in the formation of junctions between the amacrine and retinal ganglion cells.
我们在大鼠和小鼠的视网膜中发现了表达c-kit受体及其配体（干细胞因子，SLF）的细胞。通过原位杂交在大鼠和小鼠内核层的有限数量细胞中检测到了c-kit信使核糖核酸（mRNA）。基于其大小和分布模式，表达c-kit的细胞被认为是无长突细胞的一个亚群。通过使用针对小鼠c-kit受体胞外结构域的ACK2单克隆抗体，在小鼠视网膜中证实了含有c-kit蛋白的细胞。在假定的无长突细胞的细胞体和突起中检测到了c-kit蛋白。在视网膜神经节细胞中观察到了SLF mRNA的杂交信号。结果表明，c-kit受体及其配体可能在无长突细胞与视网膜神经节细胞之间连接的形成中发挥某些作用。
