Hida T, Ueda R, Sekido Y, Hibi K, Matsuda R, Ariyoshi Y, Sugiura T, Takahashi T, Takahashi T
Department of Internal Medicine, Aichi Cancer Center, Nagoya, Japan.
Int J Cancer Suppl. 1994;8:108-9. doi: 10.1002/ijc.2910570723.
Accumulating evidence suggests that c-kit plays an important role in the regulation of growth of at least 3 lineages of stem cells, while only very limited data are available on the development of human solid tumors. Our recent studies have shown that c-kit transcripts are expressed in a very restricted sub-set of human solid tumors such as small-cell lung cancer (SCLC). We have also conducted an immunohistological study on in situ localization of the c-kit protein in various human solid tumors as well as in corresponding fetal and adult normal tissues. No c-kit expression was detected in normal bronchial epithelial cells or pneumocytes in lung parenchyma of human fetal and adult specimens, indicating that the c-kit protein is aberrantly expressed in lung-cancer cells. We also found that significant chemotactic response as well as moderate in vitro cell growth occurred in SCLC cell lines upon addition of recombinant human stem-cell factor.
越来越多的证据表明，c-kit在至少3种干细胞谱系的生长调节中起重要作用，而关于人类实体瘤发生发展的可用数据却非常有限。我们最近的研究表明，c-kit转录本在人类实体瘤的一个非常有限的亚组中表达，如小细胞肺癌（SCLC）。我们还对c-kit蛋白在各种人类实体瘤以及相应的胎儿和成人正常组织中的原位定位进行了免疫组织学研究。在人类胎儿和成人标本的肺实质中的正常支气管上皮细胞或肺细胞中未检测到c-kit表达，这表明c-kit蛋白在肺癌细胞中异常表达。我们还发现，添加重组人干细胞因子后，SCLC细胞系出现显著的趋化反应以及适度的体外细胞生长。
