Watson E, Shah B, DePrince R, Hendren R W, Nelson R
Amgen, Thousand Oaks, CA.
Biotechniques. 1994 Feb;16(2):278-81.
Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.
基质辅助激光解吸电离质谱法（MALDI）已被研究作为一种表征经聚乙二醇（PEG）链共价修饰的重组干细胞因子的技术。PEG链的连接产生了分子量相差6000Da的蛋白质种类的异质混合物。这些分子量差异可以通过MALDI轻松确定。本文讨论了MALDI作为表征PEG修饰蛋白质的通用策略的潜力。
