Andersen J S, Svensson B, Roepstorff P
Department of Molecular Biology, Odense University, Denmark.
Nat Biotechnol. 1996 Apr;14(4):449-57. doi: 10.1038/nbt0496-449.
Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.
电喷雾电离和基质辅助激光解吸/电离是用于生物分子质谱分析的有效电离方法。在此，我们描述了这些方法在生物技术中用于肽和蛋白质表征的能力。本文提出了一种综合分析策略，涵盖了相应基因克隆前后的蛋白质表征。
