Ivancheva E
Acta Physiol Pharmacol Bulg. 1978;4(3):71-8.
The effect of the preincubation of mitochondria with different doses of Cu2+ on the action of sodium deoxycholate (DOC) was studied. The amount of protein released from mitochondria upon their treatment with Cu2+ as an indice for the intactness of mitochondria was studied, too. On the account of Cu2+-influence on the level of mitochondrial SH groups Cu2+-effects on the action of DOC were compared with those of a thiol reagent p-chlormercuribenzoate (p-CMB). The results obtained show the following: Cu2+ in doses higher than 20 nmoles/mg protein induces conformational changes in mitochondrial membranes becoming more pronounced over 100 nmoles Cu2+/mg protein. These changes increase mitochondrial resistance to the action of DOC. Cu2+ in doses up to 100 nmoles/mg protein depending on the protein concentration of the suspension provokes a release of lower or higher amounts of protein from mitochondria. The higher resistance of mitochondria to DOC, as well as the release of protein from mitochondria after Cu2+-incubation are not due to mercaptide binding of SH groups as p-CMB does not affect these parameters.
研究了用不同剂量的Cu2+预孵育线粒体对脱氧胆酸钠（DOC）作用的影响。还研究了用Cu2+处理线粒体后释放的蛋白质数量，以此作为线粒体完整性的指标。鉴于Cu2+对线粒体SH基团水平的影响，将Cu2+对DOC作用的影响与硫醇试剂对氯汞苯甲酸（p-CMB）的影响进行了比较。所得结果如下：剂量高于20纳摩尔/毫克蛋白质的Cu2+会诱导线粒体膜的构象变化，在Cu2+浓度超过100纳摩尔/毫克蛋白质时这种变化更加明显。这些变化增加了线粒体对DOC作用的抗性。剂量高达100纳摩尔/毫克蛋白质的Cu2+，根据悬浮液中的蛋白质浓度，会促使线粒体释放或多或少的蛋白质。线粒体对DOC的较高抗性以及Cu2+孵育后线粒体释放蛋白质，并非由于SH基团的硫醇盐结合，因为p-CMB不会影响这些参数。
