Gerritsen M J, Koopmans M J, Dekker T C, De Jong M C, Moerman A, Olyhoek T
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
Am J Vet Res. 1994 Mar;55(3):339-43.
The efficacy of dihydrostreptomycin in stopping the shedding of Leptospira hardjo subtype hardjobovis was studied in naturally infected cows. Blood and urine samples were collected from dairy cows kept on a farm where the farmer had contracted L hardjobovis infection. A microscopic agglutination test and an ELISA were used to determine specific antibody responses in serum. Polymerase chain reaction was used to detect bacterial shedding in urine. On the first sample collection date, 6 cows were seropositive, and 3 of those shed leptospires in the urine. These 3 cows were treated once with 25 mg of dihydrostreptomycin/kg of body weight. Within 1 week, the 3 cows stopped shedding leptospires. Six weeks later, 8 more lactating cows were found to be shedding leptospires. These cows were also treated once with dihydrostreptomycin, and they too stopped shedding leptospires within 1 week. From then on, the whole herd was examined weekly for a period of 2 months, and all cows Leptospira-positive by polymerase chain reaction were treated once with dihydrostreptomycin. Again, all cows stopped shedding leptospires in the urine within 1 week after treatment with dihydrostreptomycin. After a single treatment of the whole herd at the same time, new infections were not seen.
在自然感染的奶牛中研究了双氢链霉素阻止哈氏疏螺旋体哈氏牛亚种脱落的效果。从一个农场饲养的奶牛中采集血液和尿液样本，该农场主感染了哈氏牛疏螺旋体。采用显微镜凝集试验和酶联免疫吸附测定法来确定血清中的特异性抗体反应。用聚合酶链反应检测尿液中的细菌脱落情况。在首次采样日期，6头奶牛血清呈阳性，其中3头尿液中排出钩端螺旋体。这3头奶牛每千克体重用25毫克双氢链霉素治疗一次。在1周内，这3头奶牛停止排出钩端螺旋体。6周后，又发现8头泌乳奶牛排出钩端螺旋体。这些奶牛也用双氢链霉素治疗一次，它们同样在1周内停止排出钩端螺旋体。从那时起，对整个牛群每周检查2个月，所有聚合酶链反应呈钩端螺旋体阳性的奶牛都用双氢链霉素治疗一次。同样，在用双氢链霉素治疗后1周内，所有奶牛尿液中都停止排出钩端螺旋体。在对整个牛群同时进行单次治疗后，未发现新的感染情况。
