Kigasawa Hideaki, Fujiwara Masachika, Ishii Jun, Chiba Tomohiro, Terado Yuichi, Shimoyamada Hiroaki, Mochizuki Makoto, Kitamura Osamu, Kamma Hiroshi, Ohkura Yasuo
Department of Pathology, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan.
Department of Forensic Medicine, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan.
Oncol Lett. 2017 Jul;14(1):119-126. doi: 10.3892/ol.2017.6156. Epub 2017 May 11.
The multi-step progression of colorectal cancer through precancerous lesions (adenoma and dysplasia) is associated with cumulative molecular alterations, a number of which have also been demonstrated to be present in morphologically normal transitional mucosa adjacent to colorectal cancer. The cytoskeletal protein cytokeratin 7 (CK7) and the receptor tyrosine kinase, KIT proto-oncogene receptor tyrosine kinase (CD117), encoded by the proto-oncogene c-Kit, are lacking in normal colorectal crypt epithelium and are aberrantly expressed in a subset of colorectal cancer. The aim of the present study was to evaluate the expression of CK7 and CD117 in morphologically normal transitional mucosa adjacent to colorectal cancer. Immunohistochemical staining for CK7 and CD117 was performed in the mucosa adjacent to five groups of surgically resected colorectal tumors [low-grade adenoma, high-grade adenoma, mucosal adenocarcinoma, small-sized invasive adenocarcinoma (≤2 cm) and large-sized invasive adenocarcinoma (>2 cm)]. CK7 was expressed in the mucosa adjacent to a subset of colorectal tumors, and the positivity ratio increased according to tumor grade from low-grade adenoma up to small-sized invasive adenocarcinoma (61.2%). However, the positivity ratio of CK7 in the mucosa adjacent to the large-sized invasive adenocarcinoma (25.0%) was significantly lower compared with that of the next lower grade. CD117 was also expressed in the mucosa adjacent to a subset of colorectal tumors. In contrast to CK7, the positivity ratio of CD117 increased according to tumor grade from low-grade adenoma all the way through to the large-sized invasive adenocarcinoma (45.0%). Based on these results, the mechanism of CK7 and CD117 expression in the transitional mucosa adjacent to colorectal cancer may be different, and analysis of their individual expression may provide novel insights into the development and progression of colorectal cancer.
结直肠癌通过癌前病变（腺瘤和发育异常）的多步骤进展与累积的分子改变相关，其中许多分子改变也已证实在结直肠癌旁形态学正常的移行黏膜中存在。细胞骨架蛋白细胞角蛋白7（CK7）和由原癌基因c-Kit编码的受体酪氨酸激酶KIT原癌基因受体酪氨酸激酶（CD117），在正常结直肠隐窝上皮中缺乏，而在一部分结直肠癌中异常表达。本研究的目的是评估CK7和CD117在结直肠癌旁形态学正常的移行黏膜中的表达。对五组手术切除的结直肠肿瘤（低级别腺瘤、高级别腺瘤、黏膜腺癌、小尺寸浸润性腺癌（≤2 cm）和大尺寸浸润性腺癌（>2 cm））旁的黏膜进行了CK7和CD117的免疫组织化学染色。CK7在一部分结直肠肿瘤旁的黏膜中表达，阳性率从低级别腺瘤到小尺寸浸润性腺癌随肿瘤分级增加（6
