Gearon C M, Mortimer D, Chapman M G, Forman R G
Department of Obstetrics and Gynaecology, Guy's Hospital, London, UK.
Hum Reprod. 1994 Jan;9(1):77-82. doi: 10.1093/oxfordjournals.humrep.a138324.
This study investigated the use of human follicular fluid and pentoxifylline as inducers of the human sperm acrosome reaction in vitro. Motile sperm suspensions were prepared using a discontinuous Percoll gradient, preincubated for 3 h, divided into aliquots and exposed to various concentrations of non-heat-inactivated follicular fluid for 1 and 24 h and pentoxifylline for 30 min. Detection of the acrosome reaction involved the combined use of a fluorescent vital stain, H33258, and fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). A short (1 h) exposure to follicular fluid at concentrations of 50% or more, did not compromise sperm motility and significantly increased the proportion of spermatozoa having completed the acrosome reaction. Similarly, a 30 min exposure to pentoxifylline also significantly increased the proportion of spermatozoa having completed the acrosome reaction.
本研究调查了人卵泡液和己酮可可碱在体外诱导人精子顶体反应中的应用。使用不连续的Percoll梯度制备活动精子悬液，预孵育3小时，分成等份，并暴露于不同浓度的非热灭活卵泡液中1小时和24小时，以及己酮可可碱中30分钟。顶体反应的检测涉及联合使用荧光活体染色剂H33258和异硫氰酸荧光素偶联的花生凝集素（FITC-PNA）。短时间（1小时）暴露于浓度为50%或更高的卵泡液中，不会损害精子活力，且显著增加已完成顶体反应的精子比例。同样，暴露于己酮可可碱30分钟也显著增加了已完成顶体反应的精子比例。
