Tesarik J, Mendoza C, Carreras A
Centre for Reproductive Biology and Medicine, American Hospital of Paris, Neuilly Sur Seine, France.
Fertil Steril. 1992 Dec;58(6):1185-90. doi: 10.1016/s0015-0282(16)55567-8.
To determine whether the phosphodiesterase inhibitors caffeine and pentoxifylline influence the acrosome reaction in the conditions in which they are currently used as sperm movement enhancers.
The frequency of acrosome reaction occurring spontaneously in capacitating media or induced by physiological (follicular fluid [FF]) and artificial (ionophore A23187) stimuli was compared in the presence and absence of the phosphodiesterase inhibitors.
Private hospital and research laboratory.
PATIENTS, PARTICIPANTS: Patients undergoing routine semen examination before in vitro fertilization (no pathology detected) and healthy sperm donors.
None.
Percentage of acrosome-reacted sperm determined with the use of fluorescein-labeled Pisum sativum agglutinin as acrosomal stain.
Caffeine alone augmented the frequency of acrosome reaction, but this effect was not observed with pentoxifylline alone. However, pentoxifylline increased sperm responsiveness to the acrosome reaction-inducing stimuli, FF and ionophore A23187.
The promotion of spontaneous acrosome reaction may counteract the benefits from application of caffeine as motility stimulant. On the other hand, the sensitization to physiological acrosome reaction stimuli is expected to contribute to the improvement of sperm fertilizing ability by pentoxifylline and make this drug a potential candidate for the treatment of acrosome reaction anomalies.
确定磷酸二酯酶抑制剂咖啡因和己酮可可碱在目前用作精子运动增强剂的条件下是否会影响顶体反应。
在存在和不存在磷酸二酯酶抑制剂的情况下，比较在获能培养基中自发发生或由生理刺激（卵泡液[FF]）和人工刺激（离子载体A23187）诱导的顶体反应的频率。
私立医院和研究实验室。
患者、参与者：接受体外受精前常规精液检查的患者（未检测到病理情况）和健康精子捐赠者。
无。
使用荧光素标记的豌豆凝集素作为顶体染色剂测定顶体反应精子的百分比。
单独使用咖啡因可增加顶体反应的频率，但单独使用己酮可可碱未观察到这种效果。然而，己酮可可碱增加了精子对顶体反应诱导刺激物FF和离子载体A23187的反应性。
促进自发顶体反应可能会抵消将咖啡因用作运动刺激剂的益处。另一方面，预计对生理性顶体反应刺激的敏感性会有助于提高己酮可可碱对精子受精能力的改善，并使该药物成为治疗顶体反应异常的潜在候选药物。
