以重组逆转录酶为抗原，通过超敏酶免疫测定法（免疫复合物转移酶免疫测定法）检测尿液中的抗1型人类免疫缺陷病毒（HIV-1）免疫球蛋白G 。
Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.
作者信息
Hashinaka K, Hashida S, Hirota K, Saitoh A, Nakata A, Shinagawa H, Oka S, Shimada K, Ishikawa E
机构信息
Department of Biochemistry, Medical College of Miyazaki, Japan.
出版信息
J Clin Microbiol. 1994 Mar;32(3):819-22. doi: 10.1128/jcm.32.3.819-822.1994.
Abstract
Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.
摘要
以逆转录酶为抗原、β-D-半乳糖苷酶为标记，通过免疫测定法检测尿液中的抗1型人类免疫缺陷病毒免疫球蛋白G；该免疫测定法的灵敏度比之前以过氧化物酶为标记的免疫测定法高30倍。灵敏度和特异性均为100%。无症状携带者的最低信号比血清阴性受试者的最高信号高20倍。
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