免疫球蛋白G抗体捕获酶联免疫吸附测定法：一种用于检测体液中抗人类免疫缺陷病毒1型和2型抗体的通用测定法。
Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids.
作者信息
Thongcharoen P, Wasi C, Louisirirotchanakul S, Parry J, Connell J, Mortimer P
机构信息
Department of Microbiology, Siriraj Hospital, Bangkok, Thailand.
出版信息
J Clin Microbiol. 1992 Dec;30(12):3288-9. doi: 10.1128/jcm.30.12.3288-3289.1992.
Abstract
In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, respectively. This performance, achieved in a laboratory previously unfamiliar with the assay, suggests that GACELISA is a useful new epidemiological tool for the study of HIV infection, equally applicable to all three kinds of specimen.
摘要
在对55名人类免疫缺陷病毒（HIV）血清反应阳性患者和55名HIV血清反应阴性患者的干血、唾液及尿液样本进行的检测中，一种用于检测1型和2型HIV抗体的免疫球蛋白G捕获酶免疫测定法（GACELISA）分别在110份样本中得出109份正确结果、在109份样本中得出109份正确结果、在110份样本中得出109份正确结果。此前对该测定法并不熟悉的一家实验室取得了这样的检测结果，这表明GACELISA是一种用于研究HIV感染的实用新型流行病学工具，对所有这三种样本均同样适用。
相似文献
1
Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids.免疫球蛋白G抗体捕获酶联免疫吸附测定法：一种用于检测体液中抗人类免疫缺陷病毒1型和2型抗体的通用测定法。
J Clin Microbiol. 1992 Dec;30(12):3288-9. doi: 10.1128/jcm.30.12.3288-3289.1992.
2
Novel assay for the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine.用于检测未经处理的唾液和尿液中免疫球蛋白G抗人免疫缺陷病毒的新型检测方法。
J Med Virol. 1993 Oct;41(2):159-64. doi: 10.1002/jmv.1890410212.
3
Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems.唾液中的抗HIV抗体：唾液成分、检测方法及采集系统作用的评估
J Oral Pathol Med. 1996 Mar;25(3):104-7. doi: 10.1111/j.1600-0714.1996.tb00202.x.
4
Detection of antibodies to human immunodeficiency virus in vaginal secretions by immunoglobulin G antibody capture enzyme-linked immunosorbent assay: application to detection of seminal antibodies after sexual intercourse.通过免疫球蛋白G抗体捕获酶联免疫吸附测定法检测阴道分泌物中的人类免疫缺陷病毒抗体：应用于性交后精液抗体的检测。
J Clin Microbiol. 1994 May;32(5):1249-55. doi: 10.1128/jcm.32.5.1249-1255.1994.
5
New tests produced by Organon Teknika for the diagnosis of antibodies to HIV-1 and HIV-2.欧加农公司研发的用于诊断HIV-1和HIV-2抗体的新型检测方法。
Przegl Epidemiol. 1992;46(1-2):3-10.
6
Detection of anti-HIV-1 IgG antibodies in whole saliva by GACELISA and Western blot assays.采用GACELISA和蛋白质印迹法检测全唾液中的抗HIV-1 IgG抗体。
East Afr Med J. 1996 May;73(5):292-4.
7
Field evaluation of immunoglobulin G antibody capture tests for HIV-1 and HIV-2 antibodies in African serum, saliva and urine.
AIDS. 1991 Nov;5(11):1391-2. doi: 10.1097/00002030-199111000-00022.
8
Use of an acute seroconversion panel to evaluate a third-generation enzyme-linked immunoassay for detection of human immunodeficiency virus-specific antibodies relative to multiple other assays.使用急性血清转化检测组来评估第三代酶联免疫吸附测定法相对于多种其他检测方法检测人类免疫缺陷病毒特异性抗体的情况。
J Clin Microbiol. 2006 May;44(5):1856-8. doi: 10.1128/JCM.44.5.1856-1858.2006.
9
Sensitivity of HIV antibody detection in saliva.唾液中HIV抗体检测的敏感性。
Med Microbiol Immunol. 1993 Jul;182(3):147-51. doi: 10.1007/BF00190267.
10
Detection of antibodies to HIV-2 in the saliva of HIV-2-seropositive subjects.检测HIV-2血清阳性受试者唾液中的HIV-2抗体。
AIDS. 1994 Jun;8(6):849-50. doi: 10.1097/00002030-199406000-00020.
引用本文的文献
1
Urinary immunoglobulins in viral diagnosis: An overview.尿液免疫球蛋白在病毒诊断中的应用：综述。
Indian J Med Res. 2022 Jan;155(1):11-21. doi: 10.4103/ijmr.IJMR_808_18.
2
What a drop can do: dried blood spots as a minimally invasive method for integrating biomarkers into population-based research.一滴血能发挥怎样的作用：干血斑作为一种将生物标志物纳入基于人群研究的微创方法。
Demography. 2007 Nov;44(4):899-925. doi: 10.1353/dem.2007.0038.
3
Evaluation of a commercial enzyme immunoassay for HIV screening in urine.
Eur J Clin Microbiol Infect Dis. 2004 Nov;23(11):831-5. doi: 10.1007/s10096-004-1221-6. Epub 2004 Oct 8.
4
Comparison of three assays for HIV antibodies detection in urine to be applied to epidemiological setting.
Eur J Epidemiol. 1999 Jul;15(6):545-8. doi: 10.1023/a:1007575705141.
5
Human immunodeficiency virus antibody testing by enzyme-linked fluorescent and western blot assays using serum, gingival-crevicular transudate, and urine samples.使用血清、龈沟渗出液和尿液样本，通过酶联荧光和免疫印迹法进行人类免疫缺陷病毒抗体检测。
J Clin Microbiol. 1999 Apr;37(4):1100-6. doi: 10.1128/JCM.37.4.1100-1106.1999.
6
Detection of human immunodeficiency virus antibodies in oral fluids.口腔液中人类免疫缺陷病毒抗体的检测
Clin Diagn Lab Immunol. 1998 Jul;5(4):419-26. doi: 10.1128/CDLI.5.4.419-426.1998.
7
Detection of antibodies to human immunodeficiency virus type 1 in oral fluids: a large-scale evaluation of immunoassay performance.口腔液中1型人类免疫缺陷病毒抗体的检测：免疫测定性能的大规模评估。
Clin Diagn Lab Immunol. 1998 Mar;5(2):171-5. doi: 10.1128/CDLI.5.2.171-175.1998.
8
More reliable diagnosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/or serum with highly sensitive and specific enzyme immunoassay (immune complex transfer enzyme immunoassay): a review.通过高灵敏度和特异性酶免疫测定法（免疫复合物转移酶免疫测定法）检测尿液、唾液和/或血清中针对人类免疫缺陷病毒1型（HIV-1）的pol和gag蛋白的抗体IgG以及HIV-1的p24抗原，以更可靠地诊断HIV-1感染：综述
J Clin Lab Anal. 1997;11(5):267-86. doi: 10.1002/(SICI)1098-2825(1997)11:5<267::AID-JCLA5>3.0.CO;2-4.
9
Evaluation of two novel immunoassays designed to detect HIV antibodies in oral fluids.两种用于检测口腔液中HIV抗体的新型免疫测定法的评估。
J Clin Lab Anal. 1997;11(1):63-8. doi: 10.1002/(sici)1098-2825(1997)11:1<63::aid-jcla10>3.0.co;2-n.
10
Urine samples as a possible alternative to serum for human immunodeficiency virus antibody screening.尿液样本可作为血清的一种可能替代物用于人类免疫缺陷病毒抗体筛查。
Eur J Clin Microbiol Infect Dis. 1996 Oct;15(10):810-3. doi: 10.1007/BF01701524.
本文引用的文献
1
Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase.酶扩增——一种用于提供胎盘碱性磷酸酶免疫辅助测定的通用方法。
J Immunol Methods. 1985 Feb 11;76(2):389-93. doi: 10.1016/0022-1759(85)90316-3.
2
HIV surveillance by testing saliva.通过检测唾液进行艾滋病毒监测。
AIDS. 1988 Oct;2(5):369-71. doi: 10.1097/00002030-198810000-00006.
3
Sensitive assays for viral antibodies in saliva: an alternative to tests on serum.唾液中病毒抗体的灵敏检测方法：血清检测的替代方法
Lancet. 1987 Jul 11;2(8550):72-5. doi: 10.1016/s0140-6736(87)92737-1.
4
Preliminary report: accurate assays for anti-HIV in urine.
Lancet. 1990 Jun 9;335(8702):1366-9. doi: 10.1016/0140-6736(90)91245-6.
5
Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys.恒河猴体内免疫球蛋白从血浆向口腔的转移。
Immunology. 1978 Dec;35(6):923-31.
Zotero 插件