Adachi T, Kurata J, Nakao S, Murakawa M, Shichino T, Shirakami G, Shinomura T, Mori K
Department of Anesthesia, Kyoto University Hospital, Japan.
Anesth Analg. 1994 Jun;78(6):1154-7. doi: 10.1213/00000539-199406000-00023.
Nitric oxide (NO) synthase inhibitor (N omega-nitro-L-arginine methyl ester [L-NAME]) has been reported to reduce minimum alveolar anesthetic concentration (MAC) of halothane when administered intravenously (i.v.) and to reduce thermal hyperalgesia, or produce antinociception in the formalin test, when administered intracerebroventricularly (ICV) or intrathecally (IT). This study attempts to identify the site(s) in the central nervous system (CNS) where L-NAME acts to reduce the halothane MAC. For this purpose, we examined the effects of i.v., ICV, and IT administration of L-NAME on the halothane MAC in rats. In contrast to an earlier study, we did not observe any decrease in the halothane MAC after i.v. (10-30 mg/kg) administration of L-NAME. ICV (100 micrograms) and IT (100 micrograms and 1 mg) administration of L-NAME also did not alter the halothane MAC. These findings indicate that the L-arginine-NO pathway is not involved in the mechanism of action of halothane to suppress mechanical nociceptive response or in the nociceptive neural mechanism of mechanical stimulation.
据报道，一氧化氮（NO）合酶抑制剂（Nω-硝基-L-精氨酸甲酯 [L-NAME]）静脉注射时可降低氟烷的最低肺泡麻醉浓度（MAC），而脑室内（ICV）或鞘内（IT）注射时可减轻热痛觉过敏，或在福尔马林试验中产生抗伤害感受作用。本研究试图确定L-NAME在中枢神经系统（CNS）中发挥作用以降低氟烷MAC的位点。为此，我们研究了静脉注射、脑室内注射和鞘内注射L-NAME对大鼠氟烷MAC的影响。与早期研究不同，我们未观察到静脉注射（10 - 30 mg/kg）L-NAME后氟烷MAC有任何降低。脑室内注射（100微克）和鞘内注射（100微克和1毫克）L-NAME也未改变氟烷MAC。这些发现表明，L-精氨酸-NO途径不参与氟烷抑制机械性伤害感受反应的作用机制，也不参与机械刺激的伤害性神经机制。
