Sevaljević L, Petrović M, Bogojević D
Institute for Biological Research, Belgrade, Yugoslavia.
Burns. 1994 Apr;20(2):122-7. doi: 10.1016/s0305-4179(06)80008-2.
The effect of alpha 2-macroglobulin (alpha 2M) administration on the survival rate of lethally injured rats and molecular mechanisms regulating its hepatic expression after sublethal and lethal scalding were examined. Transcriptional activity of nuclei for the alpha 2M gene increased after a sublethal 20 per cent TBSA scald reaching a maximal three-fold increase by 12 h, whereas concentrations of the corresponding mRNA and protein attained the maximal nine- and 18-fold enhancements by 24 h, respectively. After the second, lethal scald, the plasma alpha 2M level increased during the first few hours, then dropped rapidly below the control value although the abundance of its mRNA was several fold enhanced. This anomaly was ascribed to inhibition of the alpha 2M mRNA translation caused by the second scald-induced disturbance of the haemodynamic equilibria. Eighty per cent of rats receiving alpha 2M prior to rescalding survived the second injury. Their recovery proceeded in parallel with normalization of the plasma volume and reactivation of the process of acute phase protein synthesis in the liver. A functional link between these events is discussed.
研究了给予α2-巨球蛋白（α2M）对致死性损伤大鼠存活率的影响，以及亚致死性和致死性烫伤后调节其肝脏表达的分子机制。在20%体表面积的亚致死性烫伤后，α2M基因的细胞核转录活性增加，在12小时时达到最大三倍增加，而相应的mRNA和蛋白质浓度分别在24小时时达到最大九倍和十八倍增强。在第二次致死性烫伤后，血浆α2M水平在最初几个小时内升高，然后迅速降至对照值以下，尽管其mRNA丰度增加了几倍。这种异常归因于第二次烫伤引起的血流动力学平衡紊乱导致的α2M mRNA翻译受到抑制。在再次烫伤前接受α2M的大鼠中，80%在第二次损伤中存活。它们的恢复与血浆容量的正常化以及肝脏急性期蛋白合成过程的重新激活同时进行。讨论了这些事件之间的功能联系。
