Sevaljević L, Glibetić M, Poznanović G, Petrović M, Matić S, Pantelić D
Institute for Biological Research, Belgrade, Yugoslavia.
Burns Incl Therm Inj. 1988 Aug;14(4):280-6. doi: 10.1016/0305-4179(88)90067-8.
Relative changes in acute-phase protein and albumin mRNAs from the liver of rats exposed to sublethal and lethal scaldings were examined by hybridization and cell-free translation. Infliction of a sublethal scalding comprising 20 per cent of the total skin area resulted in a seven-fold increase of alpha 1-acid glycoprotein (AGP), alpha 1-cysteine protease inhibitor (CPI) and the alpha- and gamma-fibrinogens' (Fb) mRNAs, whereas the level of haptoglobin (Hp) mRNA increased three times. The simultaneous infliction of two such sublethal scaldings were lethal and accompanied by a significant decrease in the concentration of AGP while the levels of CPI. Hp and Fb mRNAs remained similar to those observed after a single 20 per cent injury. A 4 h time delay between the two scaldings was also fatal and followed by an additional increase in Hp mRNA concentration whereas the levels of the other mRNAs were close to those observed after a single 20 per cent scalding. These results demonstrated that the fatal effect of the second scalding was not related to any inhibition of acute-phase reactants mRNA synthesis in the liver.
通过杂交和无细胞翻译检测了暴露于亚致死和致死性烫伤的大鼠肝脏中急性期蛋白和白蛋白mRNA的相对变化。造成占全身皮肤面积20%的亚致死性烫伤，导致α1-酸性糖蛋白（AGP）、α1-半胱氨酸蛋白酶抑制剂（CPI）以及α和γ纤维蛋白原（Fb）的mRNA增加了7倍，而触珠蛋白（Hp）mRNA水平增加了3倍。同时施加两次这样的亚致死性烫伤是致命的，且伴随着AGP浓度显著降低，而CPI、Hp和Fb的mRNA水平与单次20%损伤后观察到的水平相似。两次烫伤之间延迟4小时也是致命的，随后Hp mRNA浓度进一步增加，而其他mRNA水平接近单次20%烫伤后观察到的水平。这些结果表明，第二次烫伤的致命作用与肝脏中急性期反应物mRNA合成的任何抑制无关。
