Chatzaki E, Gallagher C J, Iles R K, Ind T E, Nouri A M, Bax C M, Grudzinskas J G
Academic Unit of Medical Oncology, Royal London Hospital, UK.
Br J Cancer. 1994 Jun;69(6):1010-4. doi: 10.1038/bjc.1994.198.
In order to examine the production of marker proteins, a reproducible method has been established for culturing purified epithelial cells from normal and malignant endometrium. We have examined the differential expression of secretory proteins using immunohistochemistry in frozen tissue sections, immunocytochemistry in cell cultures derived from the same specimens and protein assays on the culture supernatants. Placental protein 14 (PP14) was produced by normal premenopausal epithelium but not by the post-menopausal or malignant endometrial epithelium. In contrast, placental alkaline phosphatase (PLAP) was produced by endometrial cancers and the endometrial adenocarcinoma-derived cell line Ishikawa, but not by the normal endometrial epithelium. Other markers such as CA-125, which was produced by both normal and malignant endometrium but not by the cell line, and human chorionic gonadotrophin (beta-hCG), which was produced by Ishikawa cells but not by any of the fresh tissues, were less cancer specific. Placental alkaline phosphatase is a direct product of endometrial cancers that can be readily assayed in serum using this two-site assay to test its clinical usefulness in monitoring patients at risk for endometrial cancer.
为了检测标志物蛋白的产生，已经建立了一种可重复的方法来培养来自正常和恶性子宫内膜的纯化上皮细胞。我们使用免疫组织化学方法检测了冷冻组织切片中分泌蛋白的差异表达，使用免疫细胞化学方法检测了来自相同标本的细胞培养物中的分泌蛋白，并对培养上清液进行了蛋白质测定。胎盘蛋白14（PP14）由绝经前正常上皮细胞产生，但不由绝经后或恶性子宫内膜上皮细胞产生。相比之下，胎盘碱性磷酸酶（PLAP）由子宫内膜癌和子宫内膜腺癌衍生的细胞系石川细胞产生，但不由正常子宫内膜上皮细胞产生。其他标志物，如CA-125，正常和恶性子宫内膜均产生，但细胞系不产生；人绒毛膜促性腺激素（β-hCG），石川细胞产生，但新鲜组织均不产生，它们的癌症特异性较低。胎盘碱性磷酸酶是子宫内膜癌的直接产物，可以使用这种双位点测定法在血清中轻松检测，以测试其在监测子宫内膜癌高危患者中的临床实用性。
