番茄果实中酸性转化酶的细胞间定位及其cDNA的分子克隆
Intercellular localization of acid invertase in tomato fruit and molecular cloning of a cDNA for the enzyme.
作者信息
Sato T, Iwatsubo T, Takahashi M, Nakagawa H, Ogura N, Mori H
机构信息
Division of Bioproduction Science, Faculty of Horticulture, Chiba University, Japan.
出版信息
Plant Cell Physiol. 1993 Mar;34(2):263-9.
The localization of acid invertase (AI, EC 3.2.1.26) in tomato fruits was studied. AI was localized in the intercellular fraction (cell wall fraction). A cDNA encoding a wall-bound form of AI from tomato fruits was cloned and its nucleotide sequence was determined. The cloned cDNA was 2363 base pairs long and contained an open reading frame of 1908 base pairs which encoded a polypeptide of 636 amino acids. RNA blot analysis indicated that the mRNA for the acid invertase was about 2.5 kb in length. The levels of the mRNA were low at the mature green stage but increased during ripening of fruit.
对番茄果实中酸性转化酶（AI，EC 3.2.1.26）的定位进行了研究。AI定位于细胞间部分（细胞壁部分）。克隆了一个编码番茄果实中壁结合形式AI的cDNA，并测定了其核苷酸序列。克隆的cDNA长2363个碱基对，包含一个1908个碱基对的开放阅读框，编码一个636个氨基酸的多肽。RNA印迹分析表明，酸性转化酶的mRNA长度约为2.5 kb。在绿熟期mRNA水平较低，但在果实成熟过程中升高。
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