Marksteiner J, Mahata S K, Pycha R, Mahata M, Saria A, Fischer-Colbrie R, Winkler H
Neurochemical Unit, Clinic of Psychiatry, Innsbruck, Austria.
J Comp Neurol. 1994 Feb 8;340(2):243-54. doi: 10.1002/cne.903400209.
Secretoneurin is a peptide of 33 amino acids generated in brain by proteolytic processing of secretogranin II. The distribution of this newly characterized peptide was investigated by means of immunocytochemistry and in situ hybridization in the spinal cord and lower brainstem of the rat. The staining pattern of secretoneurin immunoreactivity (IR) was compared to that of substance P (SP) and calcitonin gene-related peptide (CGRP) in adjacent sections. A high density of secretoneurin-IR fibers and terminals was found in lamina I and outer lamina II of the caudal trigeminal nucleus and of the spinal cord at all levels, around the central canal, and in the sympathetic and parasympathetic areas of the lateral cell columns. The ventral horn displayed a low to moderate density of secretoneurin-IR. The highest number of secretogranin II mRNA-containing cells was found in lamina II of the dorsal horn and in neurons of the dorsal root ganglia. In the white matter, secretoneurin-IR was most prominent in the dorsolateral part of the lateral funiculus and in the tract of Lissauer. The distributions of secretoneurin-IR and SP-IR were strikingly similar. CGRP-IR and secretoneurin-IR overlapped in the outer laminae of the dorsal horn, in the lateral cell column, and probably in some motoneurons. This study establishes that, like SP and CGRP, secretoneurin is a peptide highly concentrated in the terminal field of primary afferents and in sympathetic and parasympathetic areas. Thus secretoneurin might be involved in the modulation of afferent transmission.
促分泌素原神经肽是一种由分泌粒蛋白II经蛋白水解加工在脑内产生的含33个氨基酸的肽。通过免疫细胞化学和原位杂交方法，对大鼠脊髓和低位脑干中这种新鉴定的肽的分布进行了研究。在相邻切片中，将促分泌素原神经肽免疫反应性（IR）的染色模式与P物质（SP）和降钙素基因相关肽（CGRP）的染色模式进行了比较。在尾侧三叉神经核和各级脊髓的I层和II层外层、中央管周围以及外侧细胞柱的交感和副交感区域，发现了高密度的促分泌素原神经肽-IR纤维和终末。腹角显示出低至中等密度的促分泌素原神经肽-IR。在背角II层和背根神经节神经元中发现了含分泌粒蛋白II mRNA的细胞数量最多。在白质中，促分泌素原神经肽-IR在外侧索的背外侧部分和Lissauer束中最为突出。促分泌素原神经肽-IR和SP-IR的分布惊人地相似。CGRP-IR和促分泌素原神经肽-IR在背角外层、外侧细胞柱以及可能在一些运动神经元中重叠。这项研究表明，与SP和CGRP一样，促分泌素原神经肽是一种高度集中在初级传入终末场以及交感和副交感区域的肽。因此，促分泌素原神经肽可能参与传入传递的调节。
