Cantoni O, Hussain S, Guidarelli A, Cattabeni F
Instituto di Farmacologia e Farmacognosia, Università di Urbino, Italy.
Mutat Res. 1994 Jun;324(1-2):1-6. doi: 10.1016/0165-7992(94)90059-0.
Hydrogen peroxide-resistant Chinese hamster ovary (CHO) cells displayed cross-resistance to CdCl2, HgCl2 and NaAsO2 but not to Na2Cr2O7, ZnCl2, NiCl2 and CuSO4. Resistance to hydrogen peroxide and to the metals was partially retained by these cells for many generations despite growth in drug-free medium. The loss of resistance was a slow process, and was different for the various metal compounds. Cell variants had a slightly higher content of non-protein intracellular thiols (NPSH) than sensitive cells. This biochemical feature did not seem to be the cause of resistance to CdCl2 but accounted for at least part of the resistance to HgCl2 and NaAsO2. Increased metallothionein synthesis did not seem to be responsible for the metal-resistant phenotype. These results suggest that resistance to specific metal compounds in cultured mammalian cells adapted to hydrogen peroxide is dependent on a number of factors which differ for the various metal compounds and which are characterized by a different stability.
对过氧化氢具有抗性的中国仓鼠卵巢（CHO）细胞对氯化镉、氯化汞和亚砷酸钠表现出交叉抗性，但对重铬酸钠、氯化锌、氯化镍和硫酸铜没有交叉抗性。尽管在无药物培养基中生长，但这些细胞对过氧化氢和金属的抗性在许多代中仍部分保留。抗性的丧失是一个缓慢的过程，并且因各种金属化合物而异。细胞变体的非蛋白质细胞内硫醇（NPSH）含量比敏感细胞略高。这种生化特征似乎不是对氯化镉抗性的原因，但至少部分解释了对氯化汞和亚砷酸钠的抗性。金属硫蛋白合成的增加似乎与金属抗性表型无关。这些结果表明，在适应过氧化氢的培养哺乳动物细胞中，对特定金属化合物的抗性取决于多种因素，这些因素因各种金属化合物而异，并且具有不同的稳定性。
