Cai L, Wang P, Piao X G
Institute of Radiation Medicine, Norman Bethune University of Medical Sciences, Changchun, People's Republic of China.
Mutat Res. 1994 Jun;324(1-2):13-7. doi: 10.1016/0165-7992(94)90061-2.
Cytogenetic adaptive response of mouse germ cells was studied by exposing male mice to a sequence of 4 conditioning doses of 0.05 Gy each (D1) administered at 10-day intervals and subsequently to a single challenging dose of 1.5 Gy (D2). In concurrent experiments, male mice after treatment with D1 doses alone were mated to unirradiated females and the F1 males were given the D2 dose. Chromosomal aberrations in both spermatocytes and bone-marrow cells and UV-induced UDS in splenocytes of these mice were studied. Adapted mice (i.e., D1 + D2 exposures) responded with a significantly lower frequency of chromosomal aberrations than the non-adapted (D2 exposure only) controls. The relative reduction in frequencies was, however, similar to that observed in earlier work with a single conditioning dose of 0.05 Gy. The frequencies of chromosomal aberrations in spermatocytes and bone-marrow cells as well as the levels of UV-induced UDS in splenocytes of the F1 males in the group D1 to fathers + D2 to F1 males were the same as those in F1 males which received only the D2 exposure.
通过将雄性小鼠暴露于一系列4次预处理剂量（每次0.05 Gy，D1），每隔10天给予一次，随后再给予一次1.5 Gy的单次激发剂量（D2），研究了小鼠生殖细胞的细胞遗传学适应性反应。在同期实验中，仅用D1剂量处理后的雄性小鼠与未受辐射的雌性小鼠交配，然后给F1代雄性小鼠给予D2剂量。研究了这些小鼠精母细胞和骨髓细胞中的染色体畸变以及脾细胞中紫外线诱导的非程序DNA合成（UDS）。适应性小鼠（即接受D1 + D2照射）的染色体畸变频率明显低于未适应性（仅接受D2照射）对照。然而，频率的相对降低与早期使用单次0.05 Gy预处理剂量的研究中观察到的情况相似。在D1给父亲 + D2给F1代雄性小鼠组中，F1代雄性小鼠精母细胞和骨髓细胞中的染色体畸变频率以及脾细胞中紫外线诱导的UDS水平与仅接受D2照射的F1代雄性小鼠相同。
