De Gaspari E N, Stolf A M, Umezawa E S, Zingales B, Abrahamsohn I A
Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brazil.
Acta Trop. 1994 Feb;56(1):79-87. doi: 10.1016/0001-706x(94)90042-6.
The specificity and reactivity of antibodies bound to the surface of Trypanosoma cruzi blood forms at the very early acute phase of murine infection was investigated. Surface-bound antibodies of the IgG and IgM isotypes were recovered from blood forms upon incubation at 37 degrees C. The eluted antibodies immunoprecipitated several trypomastigote surface polypeptides from 80 to 100 kDa. In contrast, for epimastigotes a very faint reactivity was detected only for antigens of 50 and 95 kDa. The shed antibodies promoted in vitro complement-mediated lysis of live blood forms and reacted with fixed trypomastigotes by immunofluorescence. Thus, blood forms are already coated with active trypomastigote-specific antibodies with a potential role in the host defense, although the low levels of serum antibodies have prevented the demonstration of humoral protection at the early stages of infection.
研究了小鼠感染急性期极早期克氏锥虫血液型表面结合抗体的特异性和反应性。在37℃孵育后，从血液型中回收了IgG和IgM同种型的表面结合抗体。洗脱的抗体免疫沉淀了几种分子量从80至100 kDa的锥鞭毛体表面多肽。相比之下，对于上鞭毛体，仅检测到对50 kDa和95 kDa抗原的非常微弱的反应性。脱落的抗体促进了活血液型的体外补体介导的裂解，并通过免疫荧光与固定的锥鞭毛体发生反应。因此，血液型已经被具有宿主防御潜在作用的活性锥鞭毛体特异性抗体所覆盖，尽管血清抗体水平较低，阻碍了在感染早期体液保护作用的证明。
