Pette M, Gengaroli C, Hartung H P, Greiner A, Giegerich G, Toyka K V
Department of Neurology (Clinical Research Unit for Multiple Sclerosis), Julius-Maximilians-Universität, Würzburg, Germany.
J Neuroimmunol. 1994 Jun;52(1):47-52. doi: 10.1016/0165-5728(94)90161-9.
In patients with inflammatory demyelinating neuropathy, which is possibly mediated by autoreactive, myelin-specific T lymphocytes, most studies focusing on immune responses to the major neuritogenic myelin protein P2 have been performed with bovine P2. However, the primary structure of bovine P2 differs from the human protein by nine amino acid residues that may profoundly influence the antigen recognition by T lymphocytes. We purified bovine and human P2 from peripheral nervous tissue and established a total of 19 T cell lines (TCL) reactive with bovine P2 from blood of two patients with acute Guillain-Barré syndrome (n = 5 TCL) and from six healthy individuals. Only four of these TCL, all raised from the blood of the GBS patients, transiently cross-recognized human P2 protein. Our results suggest that the use of human autoantigen may be crucial for the characterization of T cellular immune responses against P2 protein both in patients with inflammatory demyelinating neuropathy and in healthy controls.
在炎性脱髓鞘性神经病患者中，该病可能由自身反应性、髓鞘特异性T淋巴细胞介导，大多数针对主要致神经炎髓鞘蛋白P2免疫反应的研究都是用牛P2进行的。然而，牛P2的一级结构与人类蛋白有9个氨基酸残基不同，这可能会深刻影响T淋巴细胞对抗原的识别。我们从周围神经组织中纯化了牛P2和人P2，并建立了总共19个T细胞系（TCL），这些细胞系对两名急性吉兰-巴雷综合征患者（n = 5个TCL）和六名健康个体血液中的牛P2有反应。这些TCL中只有4个（均来自GBS患者的血液）能短暂交叉识别人类P2蛋白。我们的结果表明，使用人类自身抗原对于在炎性脱髓鞘性神经病患者和健康对照中表征针对P2蛋白的T细胞免疫反应可能至关重要。
