Weishaupt A, Giegerich G, Jung S, Gold R, Enders U, Pette M, Hayasaka K, Hartung H P, Toyka K V
Department of Neurology, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
J Neuroimmunol. 1995 Dec 31;63(2):149-56. doi: 10.1016/0165-5728(95)00139-5.
The major neuritogenic protein of peripheral nerve myelin is the P2 protein. Human P2 is a candidate autoantigen in inflammatory demyelinating diseases of the peripheral nervous system. Since human P2 is not readily available, we produced full-length recombinant human P2 protein (rhP2) in Escherichia coli. RhP2 was recognized by neuritogenic rat T cell lines and induced experimental autoimmune neuritis in Lewis rats. Production of rhP2 allowed the generation of human T cell lines reactive to the autologous protein. Studies of human T cell autoreactivity as well as efforts to use hP2 as a tolerogen will be facilitated by the large-scale expression of rhP2.
周围神经髓鞘的主要促神经突生长蛋白是P2蛋白。人P2是周围神经系统炎性脱髓鞘疾病中的候选自身抗原。由于人P2不易获得，我们在大肠杆菌中制备了全长重组人P2蛋白（rhP2）。rhP2可被促神经突生长的大鼠T细胞系识别，并在Lewis大鼠中诱发实验性自身免疫性神经炎。rhP2的制备使得能够产生对自身蛋白有反应的人T细胞系。rhP2的大规模表达将有助于对人T细胞自身反应性的研究以及将hP2用作耐受原的努力。
