Kataoka H, Nakai K, Makita M
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):909-16. doi: 10.1006/bbrc.1994.1788.
To determine how cell proliferation contributes to phosphotyrosine (P-Tyr) level in body we investigated the changes of P-Tyr levels in mouse urine and liver during liver regeneration after partial hepatectomy (PH). After PH, the P-Tyr contents in urine and liver increased approximately 14- and 1.7-fold, respectively, at 2-4 days, and P-Tyr phosphatase activity in liver decreased to 50% at 2 days. These levels returned to the control level at 10 days. After orthovanadate administration, P-Tyr level increased as P-Tyr phosphatase activity decreased. These results suggest that P-Tyr level in liver is reflected in urinary P-Tyr level during liver regeneration, and P-Tyr phosphatase is important in the regulation of P-Tyr level.
为了确定细胞增殖如何影响机体中的磷酸酪氨酸（P-Tyr）水平，我们研究了部分肝切除（PH）后肝脏再生过程中小鼠尿液和肝脏中P-Tyr水平的变化。PH后，尿液和肝脏中的P-Tyr含量在2 - 4天时分别增加了约14倍和1.7倍，肝脏中的P-Tyr磷酸酶活性在2天时降至50%。这些水平在10天时恢复到对照水平。给予原钒酸盐后，P-Tyr水平随着P-Tyr磷酸酶活性的降低而升高。这些结果表明，肝脏再生过程中肝脏中的P-Tyr水平反映在尿液P-Tyr水平中，并且P-Tyr磷酸酶在P-Tyr水平的调节中起重要作用。
