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黑眶蟾蜍排卵前及排卵期间输卵管的黏多糖组织化学

Mucopolysaccharide histochemistry of the oviduct of the toad, Bufo melanostictus, before and during ovulation.

作者信息

Tan C K, Chen T W, Gan J M

机构信息

Department of Anatomy, National University of Singapore, Kent Ridge.

出版信息

Histol Histopathol. 1994 Jan;9(1):113-8.

PMID:7516206
Abstract

In both non-ovulating and ovulating toads of the species, Bufo melanostictus, the fimbrium stained only weakly for mucopolysaccharides (MPs) whereas the infundibulum stained strongly for neutral MPs and also for glycogen. In the non-ovulating toad, only neutral and sulphated MPs were detected in the goblet cells of the upper magnum, whereas sulphated, neutral and sialomucins were detected in the glands. In the middle magnum, sulphated and sialic acid-containing carboxylated MPs were detected in both the goblet cells and glands. In the lower magnum, neutral, sulphated, and sialic acid-containing MPs were detected in the goblet cells and only sulphated and sialic acid-containing MPs were detected in the glands. In the isthmus and ovisac, only sulphated MPs were present in the goblet cells. During ovulation, there was no change in the distribution of sulphated MPs throughout the oviduct. Sialic acid-containing MPs could not be detected in many of the goblet cells of the upper and lower magnum nor in most of the glands of the lower magnum.

摘要

在黑眶蟾蜍(Bufo melanostictus)的非排卵和排卵蟾蜍中,伞部对黏多糖(MPs)的染色仅呈弱阳性,而漏斗部对中性MPs和糖原的染色呈强阳性。在非排卵蟾蜍中,仅在上段输卵管的杯状细胞中检测到中性和硫酸化MPs,而在腺体中检测到硫酸化、中性和唾液酸黏蛋白。在中段输卵管中,在杯状细胞和腺体中均检测到硫酸化和含唾液酸的羧化MPs。在下端输卵管中,在杯状细胞中检测到中性、硫酸化和含唾液酸的MPs,而在腺体中仅检测到硫酸化和含唾液酸的MPs。在峡部和卵囊,杯状细胞中仅存在硫酸化MPs。在排卵期间,整个输卵管中硫酸化MPs的分布没有变化。在上段和下段输卵管的许多杯状细胞以及下段输卵管的大多数腺体中均未检测到含唾液酸的MPs。

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