Méndez B A, Moreno A R, Olavarría J M
Acta Physiol Lat Am. 1976;26(2):122-37.
A histo-biochemical correlation was carried out in oviducts of Bufo arenarum (Hensel), in trophic animals in the preovulatory period. Histochemical techniques were used to detect acidic mucoproteins, whereas complex mucopolysaccharides were biochemically assayed, thus simplifying the histologic methodology. The existence of acetylhexosamines, hexoses, phosphate and sialic acid in the oviduct was put in evidence through histochemical and biochemical methods. Results show a high content in sialic aicd, total phosphate and acetylhexosamines in the middle and terminal portions of the preconvoluted zone. Sialomucins, acetylhexosamines and hexosamines show a maximum in the last loops of the convoluted zone, coincident with clearly determined histologic areas. All components detected exhibit minimal values in the proximal and distal intermediate zones. Results found for phosphate show it is combined, and determines the reactivity to the stains used. This type of mucin has not been found in other species.
对处于排卵前期的营养期动物——阿根廷蟾蜍（亨塞尔）的输卵管进行了组织生化相关性研究。采用组织化学技术检测酸性粘蛋白，而对复合粘多糖进行生化分析，从而简化了组织学方法。通过组织化学和生化方法证实了输卵管中存在乙酰己糖胺、己糖、磷酸盐和唾液酸。结果显示，在盘曲前区的中部和末端部分，唾液酸、总磷酸盐和乙酰己糖胺含量较高。唾液粘蛋白、乙酰己糖胺和己糖胺在盘曲区的最后几圈达到最大值，与明确确定的组织学区域一致。在近端和远端中间区，所有检测到的成分含量均最低。磷酸盐的检测结果表明其处于结合状态，并决定了对所用染色剂的反应性。这种类型的粘蛋白在其他物种中尚未发现。
