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BrdU 替代染色体中姐妹染色单体的差异碳酸银染色

Differential silver carbonate staining of sister chromatids in BrdU-substituted chromsomes.

作者信息

Goyanes V J

出版信息

Hum Genet. 1978 Jan 19;40(2):205-8. doi: 10.1007/BF00272302.

DOI:10.1007/BF00272302
PMID:75163
Abstract

Differential staining of sister chromatids in BrdU-substituted human chromosomes is demonstrated by an ammoniacal silver carbonate procedure. With this method the chromosomes exhibit a subchromatid structure. Because proteolytic treatment indicated that the silver carbonate binds the chromosome proteins, changes of these components may be inferred in the BrdU-substituted chromosomes. Sister chromatid exchanges could be identified.

摘要

通过氨性碳酸银法可对BrdU取代的人类染色体中的姐妹染色单体进行差异染色。用这种方法，染色体呈现出亚染色单体结构。由于蛋白水解处理表明碳酸银与染色体蛋白结合，因此可以推断出BrdU取代的染色体中这些成分的变化。可以识别姐妹染色单体交换。

相似文献

1
Differential silver carbonate staining of sister chromatids in BrdU-substituted chromsomes.BrdU 替代染色体中姐妹染色单体的差异碳酸银染色
Hum Genet. 1978 Jan 19;40(2):205-8. doi: 10.1007/BF00272302.
2
Opposite staining effect of two silver-staining techniques on sister chromatids.两种银染技术对姐妹染色单体的相反染色效果。
Exp Cell Res. 1985 Jul;159(1):55-62. doi: 10.1016/s0014-4827(85)80037-9.
3
Electron microscopy of sister chromatid exchanges.姐妹染色单体交换的电子显微镜检查。
Cytogenet Cell Genet. 1983;36(4):612-6. doi: 10.1159/000131984.
4
Mechanisms involved in differential staining of BrdU substituted chromatids or chromosome regions.BrdU 取代染色单体或染色体区域差异染色所涉及的机制。
Rom J Morphol Embryol. 1995 Jan-Jun;41(1-2):43-7.
5
A method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold-silver staining.一种利用早熟凝集染色体和免疫金银染色检测姐妹染色单体交换的方法。
Mutagenesis. 1997 May;12(3):175-7. doi: 10.1093/mutage/12.3.175.
6
Reverse differential staining of sister chromatids induced by Hoechst plus black light and endonuclease.由Hoechst加黑光和核酸内切酶诱导的姐妹染色单体反向差别染色
Exp Cell Res. 1985 Apr;157(2):307-14. doi: 10.1016/0014-4827(85)90115-6.
7
Atomic force microscopy of human metaphase chromosomes after differential staining of sister chromatids.姐妹染色单体差异染色后人类中期染色体的原子力显微镜观察。
Arch Histol Cytol. 2004 Jun;67(2):171-7. doi: 10.1679/aohc.67.171.
8
An analysis of factors for the differential staining of sister chromatids in human chromosomes using Giemsa.使用吉姆萨染色法对人类染色体中姐妹染色单体差异染色的因素分析。
Acta Genet Med Gemellol (Roma). 1978;27:81-7. doi: 10.1017/s0001566000009545.
9
The role of chromosomal proteins in the induction of a differential staining of sister chromatids by light.染色体蛋白在光诱导姐妹染色单体差异染色中的作用。
Histochem J. 1981 Sep;13(5):735-46. doi: 10.1007/BF01003286.
10
Labelling of DNA and differential sister chromatid staining after BrdU treatment in vivo.体内经BrdU处理后的DNA标记及姐妹染色单体差异染色
Chromosoma. 1976 Aug 4;57(1):13-8. doi: 10.1007/BF00292946.

引用本文的文献

1
Sequential staining of euchromatic and heterochromatic regions of the human Y chromosome.人类Y染色体常染色质区和异染色质区的顺序染色
J Med Genet. 1980 Dec;17(6):468-71. doi: 10.1136/jmg.17.6.468.
2
Contradictory differential staining results with Coomassie Brilliant Blue and silver carbonate on sister chromatids.
Experientia. 1982 Jul 15;38(7):853-4. doi: 10.1007/BF01972313.
3
Differential gene activity visualized on sister chromatids after replication in the presence of 5-azacytidine.在5-氮杂胞苷存在的情况下复制后，在姐妹染色单体上可视化的差异基因活性。

本文引用的文献

1
Differential spiralization along mammalian mitotic chromosomes. I. BUdR-revealed differentiation in Chinese hamster chromosomes.哺乳动物有丝分裂染色体上的差异螺旋化。I. 用5-溴脱氧尿苷显示中国仓鼠染色体的差异
Chromosoma. 1972;38(4):341-65. doi: 10.1007/BF00320156.
2
Giemsa technique for the detection of sister chromatid exchanges.用于检测姐妹染色单体交换的吉姆萨技术。
Chromosoma. 1974;48(4):355-60. doi: 10.1007/BF00290992.
3
[Chromatid exchanges in human mitotic cells. BUDR Treatment and bichromatic fluorescence by aridine orange (author's transl)].
Chromosoma. 1985;91(3-4):307-12. doi: 10.1007/BF00328226.
[人类有丝分裂细胞中的染色单体交换。BUDR处理及吖啶橙双色荧光法（作者译）]
Chromosoma. 1974;48(3):327-40. doi: 10.1007/BF00326509.
4
New Giemsa method for the differential staining of sister chromatids.用于姐妹染色单体鉴别染色的新吉姆萨方法。
Nature. 1974 Sep 13;251(5471):156-8. doi: 10.1038/251156a0.
5
Spontaneous sister chromatid exchanges detected by a BUdR-labelling method.采用5-溴脱氧尿嘧啶核苷（BUdR）标记法检测自发的姐妹染色单体交换。
Nature. 1974 Sep 6;251(5470):70-2. doi: 10.1038/251070a0.
6
Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C.姐妹染色单体交换，人类染色体损伤与修复的指标：通过荧光检测及丝裂霉素C诱导
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3162-6. doi: 10.1073/pnas.71.8.3162.
7
Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes.光脉冲对5-溴脱氧尿苷和5-碘脱氧尿苷取代的中国仓鼠染色体诱导的姐妹染色单体交换
Exp Cell Res. 1974 Jul;87(1):15-9. doi: 10.1016/0014-4827(74)90521-7.
8
Mechanisms of chromosome banding. II. Evidence that histones are not involved.染色体显带机制。II. 组蛋白不参与的证据。
Exp Cell Res. 1974 May;86(1):202-6. doi: 10.1016/0014-4827(74)90674-0.
9
Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.人中期染色体中脱氧核糖核酸复制的显微荧光检测
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9. doi: 10.1073/pnas.70.12.3395.
10
Effects of treatment on differential staining of BrdU labeled metaphase chromosomes: three-way differentiation of M3 chromosomes.治疗对BrdU标记中期染色体差异染色的影响：M3染色体的三向分化
Chromosoma. 1976 Mar 31;55(1):1-11. doi: 10.1007/BF00288322.