一种利用早熟凝集染色体和免疫金银染色检测姐妹染色单体交换的方法。

A method for detecting sister chromatid exchanges using prematurely condensed chromosomes and immunogold-silver staining.

作者信息

Asakawa Y, Gotoh E

机构信息

Division of Genetic Resources, National Institute of Health Japan, Shinjuku-ku, Tokyo, Japan.

出版信息

Mutagenesis. 1997 May;12(3):175-7. doi: 10.1093/mutage/12.3.175.

DOI:10.1093/mutage/12.3.175

PMID:9175644

Abstract

We have developed a method for detecting sister chromatid exchanges using premature chromosome condensation and immunogold labelling followed by silver enhancement. Cells were grown in bromodeoxyuridine before sister chromatid exchanges were generated with mitomycin C. Calyculin A was then used to condense the chromosomes prematurely. Finally, incorporated bromodeoxyuridine was detected using a gold-conjugated antibody followed by silver enhancement. Exchanged chromatids could easily be seen in the resulting chromosomes. This simple method allows sister chromatid exchanges to be detected with great sensitivity and resolution.

摘要

我们开发了一种利用早熟染色体凝聚、免疫金标记及随后的银增强来检测姐妹染色单体交换的方法。在使用丝裂霉素C产生姐妹染色单体交换之前，细胞在溴脱氧尿苷中培养。然后使用花萼海绵诱癌素A使染色体过早凝聚。最后，使用金标抗体检测掺入的溴脱氧尿苷，随后进行银增强。在所得染色体中可以很容易地看到交换的染色单体。这种简单的方法能够以高灵敏度和分辨率检测姐妹染色单体交换。