Andrews P M
Department of Anatomy and Cell Biology, Georgetown University Medical Center, Washington, DC 20007.
Transplantation. 1994 Jun 15;57(11):1567-75.
In this study, the ability of low molecular dextrans to prevent morphologically detectable acute tubular necrosis during cold storage was evaluated. Rat kidneys were flushed with a sodium phosphate buffer (pH 7.2) containing different concentrations of dextran 10 (m.w. of 10,000 or less) and stored at 0-2 degrees C for up to 5 days (samples taken at 24-hr intervals). It was found that solutions containing 20% or more of dextran 10 provided significantly improved morphological preservation of kidney nephrons when compared with currently popular kidney cold storage preservation solutions (i.e. University of Wisconsin and Euro-Collins solutions). Adding smaller amounts (i.e., 15%) of dextran 10 to a cold storage solution already containing another effective osmotic agent (i.e., sucrose) also resulted in superior morphological preservation, indicating a beneficial additive effect of using more than one osmotic agent. Dextran 40 (m.w. 40,000) did not provide as good morphological preservation as did a similar concentration of dextran 10. It is concluded that the use of the proper kind and proper amount of low molecular weight dextrans in preservation solutions can significantly reduce the morphologically detectable acute tubular necrosis during cold storage.
在本研究中，评估了低分子右旋糖酐在冷藏期间预防形态学上可检测到的急性肾小管坏死的能力。用含有不同浓度右旋糖酐10（分子量为10,000或更低）的磷酸钠缓冲液（pH 7.2）冲洗大鼠肾脏，并在0-2℃下储存长达5天（每隔24小时取样）。结果发现，与目前常用的肾脏冷藏保存液（即威斯康星大学溶液和欧洲柯林斯溶液）相比，含有20%或更多右旋糖酐10的溶液能显著改善肾单位的形态学保存。向已含有另一种有效渗透剂（即蔗糖）的冷藏溶液中添加较少量（即15%）的右旋糖酐10，也能实现更好的形态学保存，这表明使用多种渗透剂具有有益的相加作用。右旋糖酐40（分子量40,000）在形态学保存方面不如相同浓度的右旋糖酐10。结论是，在保存液中使用合适种类和合适量的低分子右旋糖酐可显著减少冷藏期间形态学上可检测到的急性肾小管坏死。
