Cybulski Z, Michalska W, Pietkiewicz K
Department of Medical Microbiology, K. Marcinkowski Medical Academy, Poznań, Poland.
Acta Microbiol Pol. 1993;42(3-4):259-65.
117 P. aeruginosa strains have been isolated from hospital material (vascular ward), from ambulatory patients, from lake water samples and from animal environment. The serological characters of the above strains, their phage--typing patterns, their capability of producing ONPG hydrolase were compared in order to find out the strain with identical features responsible for nosocomial infections and also to find endemic infections. There were eleven polyagglutinable strains (isolated from sinks in the vascular ward) which agglutinated with two sera and one strain isolated from lake water. Apart from one exception there were no confirmations of the occurrence of the strains of identical features in different environments. Possible variability within the scope of somatic antigen and the phage typing of microorganism confirm the necessity to use several techniques to carry out studies of epidemiological significance.
从医院材料(血管病房)、门诊患者、湖水样本和动物环境中分离出了117株铜绿假单胞菌菌株。对上述菌株的血清学特征、噬菌体分型模式、产生邻硝基苯-β-D-半乳糖苷(ONPG)水解酶的能力进行了比较,以便找出导致医院感染且具有相同特征的菌株,并找出地方性感染。有11株多凝集性菌株(从血管病房的水槽中分离出)与两种血清发生凝集反应,还有1株从湖水中分离出的菌株。除了一个例外情况外,未证实在不同环境中存在具有相同特征的菌株。微生物体细胞抗原范围和噬菌体分型内可能存在的变异性证实了有必要使用多种技术来开展具有流行病学意义的研究。