Demina N S
Biol Bull Acad Sci USSR. 1978 May-Jun;5(3):281-8.
Literature material is presented on the ability of free-living nodule bacteria for asymbiotic nitrogen fixation, detected for the first time in 1975. Necessary components of the nutrient medium in the use of which the nitrogen-fixing ability of rhizobia in pure cultures is manifested, proved to be sugars and intermediates of the citric acid cycle, as well as small quantities of bound nitrogen. The experimental data available in the literature are evidence of the presence of a complete assortment of genes for the synthesis of the nitrogenase enzyme complex in free-living nodule bacteria.
文献资料介绍了1975年首次检测到的自生根瘤菌的非共生固氮能力。已证明,在纯培养物中表现根瘤菌固氮能力时,营养培养基的必要成分是糖类、柠檬酸循环的中间产物以及少量的结合态氮。文献中的实验数据证明自生根瘤菌中存在合成固氮酶复合物的全套基因。