Krishchenko V P
Biol Bull Acad Sci USSR. 1978 May-Jun;5(3):310-9.
In the proposed method, the determination of the amount and amino acid composition of extractable and inextractable proteins, nonprotein nitrogen, and free amino acids is performed in one analytical sample. The above-mentioned nitrogenous substances are extracted with borate buffer. Shortened and expanded schemes of separation of proteins into fractions using buffer solutions are given. For the shortened schemes of analysis, the proteins are separated into readily soluble, medium soluble, sparingly soluble, and insoluble fractions, and in the expanded scheme into eight fractions: three fractions of readily soluble proteins, two fractions each of medium- and sparingly-soluble proteins, and insoluble proteins.
在所提出的方法中，在一个分析样品中测定可提取和不可提取蛋白质、非蛋白氮和游离氨基酸的含量及氨基酸组成。上述含氮物质用硼酸盐缓冲液提取。给出了使用缓冲溶液将蛋白质分离成组分的简化和扩展方案。对于简化分析方案，蛋白质被分离成易溶、中等溶解度、微溶和不溶组分，而在扩展方案中则分离成八个组分：三个易溶蛋白质组分、两个中等溶解度和微溶蛋白质组分以及不溶蛋白质组分。
