Effects of activin A on ionic channels were examined in human FSH-secreting tumour cells using electrophysiological techniques. 2. Under voltage clamp with the conventional whole-cell clamp technique, the voltage-gated Na+ channel, the T- and L-type Ca2+ channels, the delayed K+ channel and the A-channel were observed. 3. With the nystatin-perforated whole-cell clamp technique, the same voltage-gated channels were recorded. Activin A (10(-7) M) increased the amplitude of the L-type Ca2+ current, whereas it decreased the amplitude of the delayed K+ current. 4. Under current clamp with the perforated whole-cell clamp technique, more than 80% of the cells exhibited spontaneous action potentials. Application of 10(-7) M activin A depolarized the membrane with a conductance increase and augmented action potential frequency. The reversal potential of the activin A-induced current was -20 to 0 mV. The activin A-induced current was abolished in a Na(+)-free extracellular solution, indicating that the membrane depolarization caused by activin A was due to the conductance increase to Na+ ions through non-selective cation channels.
