鳄鱼某些背侧丘脑核中的谷氨酸脱羧酶免疫反应性
Glutamic acid decarboxylase immunoreactivity in some dorsal thalamic nuclei in Crocodilia.
作者信息
Pritz M B, Stritzel M E
机构信息
Department of Neurological Surgery, California College of Medicine, University of California Irvine Medical Center, Orange 92668.
出版信息
Neurosci Lett. 1994 Jan 3;165(1-2):109-12. doi: 10.1016/0304-3940(94)90721-8.
Glutamic acid decarboxylase (GAD) immunocytochemical properties of thalamic nuclei known to project to the telencephalon were investigated in reptiles, Caiman crocodilus and Alligator mississippiensis, by monoclonal antibodies to GAD epitopes designated as GAD-1, GAD-2 and GAD-5. GAD-immunoreactive puncta were observed with all three monoclonal antibodies in the following dorsal thalamic nuclei by avidin-biotin complex methodology: dorsolateralis anterior, dorsomedialis anterior, diagonalis, rotundus, reuniens pars centralis and pars diffusa, and the medialis complex. In general, immunoreactivity to GAD was more robust in Alligator than in Caiman. GAD-2 immunoreactivity was more intense than immunoreactivity to GAD-1 or GAD-5 at similar antibody concentrations in both species. Thalamic nuclei varied in the pattern and intensity of GAD (+)puncta staining in Caiman and Alligator. No GAD-immunoreactive neurons were observed in any of these seven thalamic nuclei with any GAD antibody in either species.
利用针对谷氨酸脱羧酶（GAD）表位的单克隆抗体GAD - 1、GAD - 2和GAD - 5，对凯门鳄和密西西比鳄这两种爬行动物中已知投射至端脑的丘脑核团的GAD免疫细胞化学特性进行了研究。采用抗生物素蛋白 - 生物素复合物方法，在以下背侧丘脑核团中用这三种单克隆抗体均观察到了GAD免疫反应性小点：前背外侧核、前背内侧核、斜角核、圆核、中央联合部和弥散部以及内侧复合体。总体而言，短吻鳄中对GAD的免疫反应性比凯门鳄更强。在两种动物中，当抗体浓度相似时，GAD - 2的免疫反应性比GAD - 1或GAD - 5的免疫反应性更强。凯门鳄和短吻鳄的丘脑核团在GAD（+）小点染色的模式和强度上存在差异。在这两种动物的这七个丘脑核团中，使用任何一种GAD抗体均未观察到GAD免疫反应性神经元。
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