Department of Biomedical Engineering, University of Utah, Salt Lake City, Utah, USA.
DENLABS, Draper, Utah, USA.
Neuroreport. 2024 Oct 2;35(14):921-924. doi: 10.1097/WNR.0000000000002082. Epub 2024 Jul 26.
The objective is to determine the distribution of glutamic acid decarboxylase (GAD) in the olfactory bulb of a crocodilian, Caiman crocodilus . Avidin-biotin immunohistochemical methodology using a polyclonal antibody to GAD raised in sheep was employed. The following controls were used: substitution of the primary antibody with preimmune sheep serum at concentrations equal to that of the primary antibody; omission of the primary antibody; and omission of the primary antibody and biotinylated rabbit antisheep immunoglobulin. No GAD (+) cells were observed in the control sections. Based on cell and fiber staining, the layering and neuronal organization of the olfactory bulb in Caiman were similar to other vertebrates, including other reptiles. The following elements were GAD (+): granule cells, certain neurons in the outer plexiform layer, periglomerular neurons, and the glomeruli themselves. GAD (+) puncta were present throughout the olfactory bulb. In conclusion, these results in Caiman were similar, in part, to comparable studies in mammals and birds. Taken together, these data indicate that crocodiles not only have a similar pattern of layers that other amniotes possess but also that the immunocytochemical signatures of certain elements of the olfactory bulb are likewise shared.
目的是确定鳄鱼嗅球中谷氨酸脱羧酶（GAD）的分布。使用针对羊源 GAD 产生的多克隆抗体，采用亲和素-生物素免疫组织化学方法。采用了以下对照：用与初级抗体等浓度的抗羊血清替代初级抗体；省略初级抗体；以及省略初级抗体和生物素化兔抗羊免疫球蛋白。在对照部分未观察到 GAD（+）细胞。基于细胞和纤维染色，凯门鳄嗅球的分层和神经元组织与其他脊椎动物相似，包括其他爬行动物。以下元素为 GAD（+）：颗粒细胞、外丛状层中的某些神经元、肾小球旁神经元和肾小球本身。GAD（+）的点状结构存在于整个嗅球中。总之，这些在凯门鳄中的结果部分与哺乳动物和鸟类的可比研究相似。综上所述，这些数据表明，鳄鱼不仅具有与其他羊膜动物相似的层状结构模式，而且嗅球某些元素的免疫细胞化学特征也相同。
