Grozdanovic Z, Mayer B, Baumgarten H G, Brüning G
Department of Anatomy, Free University of Berlin, Germany.
Neuroreport. 1994 Mar 21;5(7):837-40. doi: 10.1097/00001756-199403000-00025.
We investigated the distribution pattern of nitric oxide (NO) synthesizing nerve cell bodies and axons in the biliary system of the guinea-pig using immunohistochemistry for nitric oxide synthase (NOS). Nerve fibres staining for NOS were found to contact non-vascular smooth myocytes and to course beneath the epithelium. No perivascular NOS fibres were observed. The innervation density varied in different parts of the biliary tree. The lower portion of the common bile duct was more richly innervated than the remaining parts of the duct system. NOS-containing neurones encompassed a subpopulation of intramural ganglion cells. Sympathetic neurones in the coeliac ganglion were not stained. It is suggested that intrinsic NOergic neurones are involved in inhibitory motor control of the biliary musculature, including the sphincter of Oddi.
我们运用一氧化氮合酶（NOS）免疫组织化学方法，研究了豚鼠胆道系统中合成一氧化氮（NO）的神经细胞体和轴突的分布模式。发现经NOS染色的神经纤维与非血管平滑肌细胞接触，并在上皮下方走行。未观察到血管周围的NOS纤维。胆道树不同部位的神经支配密度各不相同。胆总管下段的神经支配比管道系统的其余部分更为丰富。含NOS的神经元包括壁内神经节细胞的一个亚群。腹腔神经节中的交感神经元未被染色。提示内源性NO能神经元参与包括Oddi括约肌在内的胆道肌肉组织的抑制性运动控制。
