Narberhaus F, Pich A, Bahl H
Institut für Mikrobiologic, Georg-August-Universität Göttingen, Germany.
Curr Microbiol. 1994 Jul;29(1):13-8. doi: 10.1007/BF01570185.
The response to heat stress was examined in Thermoanaerobacterium thermosulfurigenes EM1. Upon a temperature shift-up from 50 degrees to 62 degrees C, four heat shock proteins (hsps) were synthesized at an elevated level. Two proteins were found to be immunologically related to the Escherichia coli GroEL protein and the Mycobacterium tuberculosis hsp71 (DnaK similar protein), and the corresponding groE and dnaK homologous sequences were detected in the chromosome of T. thermosulfurigenes EM1. The heat shock response in this thermophile was transient, with a maximum synthesis of hsps between 10 and 15 min after the shock. The enhanced synthesis of DnaK and GroEL was consistent with increased mRNA levels of the genes, which reached a maximum 15 min after heat treatment.
对嗜热栖热放线菌EM1中的热应激反应进行了研究。当温度从50摄氏度升至62摄氏度时，四种热休克蛋白（hsps）的合成水平升高。发现两种蛋白与大肠杆菌GroEL蛋白和结核分枝杆菌hsp71（DnaK类似蛋白）存在免疫相关性，并且在嗜热栖热放线菌EM1的染色体中检测到了相应的groE和dnaK同源序列。该嗜热菌中的热休克反应是短暂的，休克后10至15分钟时热休克蛋白的合成达到最大值。DnaK和GroEL的合成增强与基因mRNA水平的增加一致，mRNA水平在热处理后15分钟达到最大值。
