Trent J D, Osipiuk J, Pinkau T
Max-Planck-Institut für Biochemie, Munich, Federal Republic of Germany.
J Bacteriol. 1990 Mar;172(3):1478-84. doi: 10.1128/jb.172.3.1478-1484.1990.
The extreme thermophile Sulfolobus sp. strain B12 exhibits an acquired thermotolerance response. Thus, survival of cells from a 70 degrees C culture at the lethal temperature of 92 degrees C was enhanced by as much as 6 orders of magnitude over a 2-h period if the culture was preheated to 88 degrees C for 60 min or longer before being exposed to the lethal temperature. In eubacteria and eucaryotes, acquired thermotolerance correlates with the induced synthesis of a dozen or so proteins known as heat shock proteins. In this Sulfolobus species, it correlates with the preferential synthesis of primarily one major protein (55 kilodaltons) and, to a much lesser extent, two minor proteins (28 and 35 kilodaltons). Since the synthesis of all other proteins was radically reduced and these proteins were apparently not degraded or exported, their relative abundance within the cell increased during the time the cells were becoming thermotolerant. They could not yet be related to known heat shock proteins. In immunoassays, they were not cross-reactive with antibodies against heat shock proteins from Escherichia coli (DnaK and GroE), which are highly conserved between eubacteria and eucaryotes. However, it appears that if acquired thermotolerance depends on the synthesis of protective proteins, then in this extremely thermophilic archaebacterium it depends primarily on one protein.
嗜热栖热菌菌株B12表现出一种获得性耐热反应。因此，如果在暴露于致死温度之前将70℃培养的细胞在88℃预热60分钟或更长时间，那么在2小时内，细胞在92℃致死温度下的存活率会提高多达6个数量级。在真细菌和真核生物中，获得性耐热性与诱导合成十几种被称为热休克蛋白的蛋白质有关。在这种嗜热栖热菌中，它与主要一种主要蛋白质（55千道尔顿）以及程度小得多的两种次要蛋白质（28和35千道尔顿）的优先合成有关。由于所有其他蛋白质的合成急剧减少，并且这些蛋白质显然没有被降解或输出，因此在细胞变得耐热的过程中，它们在细胞内的相对丰度增加。它们还不能与已知的热休克蛋白相关联。在免疫测定中，它们与针对大肠杆菌热休克蛋白（DnaK和GroE）的抗体没有交叉反应，这些热休克蛋白在真细菌和真核生物之间高度保守。然而，似乎如果获得性耐热性取决于保护性蛋白质的合成，那么在这种极端嗜热的古细菌中，它主要取决于一种蛋白质。
