Ihara T, Yasuda N, Isaji M, Torigoe S, Ito M, Kamiya H, Sakurai M
Department of Pediatrics, Mie National Hospital, Japan.
Leuk Res. 1994 Jul;18(7):485-91. doi: 10.1016/0145-2126(94)90086-8.
To determine the role of cell-mediated immunity (CMI) to cytomegalovirus (CMV) in leukemic children after CMV infection, CMI to CMV antigen was studied using CMV-specific lymphocyte blastogenic responses (LBR) and interferon (IFN) production. Four children, who continuously secreted CMV in urine more than 2 years after symptomatic CMV infection (CMV disease) (group 1), showed impaired LBR to CMV antigen, though they had normal LBR to phytohemagglutinin (PHA) and concanavalin A (Con A). Impairment of LBR either to AD-169 strains or autologous and heterologous wild strains was observed. IFN production was not detected in three of four children. Six leukemic children, who had no viruria after cessation of CMV disease (group 2), showed good responses to CMV antigens. IFN was detected in all six children in group 2. Eight leukemic children, who were seropositive to CMV at the onset of leukemia (group 3), showed good responses to CMV antigens and IFN production. These results suggest that impaired cell-mediated immunity to CMV antigen might contribute to prolonged excretion of CMV in urine in leukemic children.
为确定细胞介导免疫（CMI）对巨细胞病毒（CMV）在CMV感染后白血病儿童中的作用，利用CMV特异性淋巴细胞增殖反应（LBR）和干扰素（IFN）产生情况研究了对CMV抗原的CMI。4名有症状的CMV感染（CMV病）后连续2年以上尿中持续分泌CMV的儿童（第1组），对CMV抗原的LBR受损，尽管他们对植物血凝素（PHA）和刀豆球蛋白A（Con A）的LBR正常。观察到对AD - 169株或自体及异源野生株的LBR均受损。4名儿童中有3名未检测到IFN产生。6名CMV病停止后无病毒尿的白血病儿童（第2组），对CMV抗原反应良好。第2组的所有6名儿童均检测到IFN。8名白血病发病时CMV血清学阳性的儿童（第3组），对CMV抗原反应良好且有IFN产生。这些结果提示，对CMV抗原的细胞介导免疫受损可能导致白血病儿童尿中CMV排泄延长。
