Beekhuizen H, van Furth R
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
J Vasc Res. 1994 Jul-Aug;31(4):230-9. doi: 10.1159/000159048.
The morphological and growth characteristics of human macrovascular endothelial cells (ECs) from venous and arterial umbilical cord vessels and microvascular ECs from foreskin were compared during cultivation. By means of time-lapse microcinematography and phase-contrast microscopy, differences in cell morphology and migratory activity between the different types of ECs were found. Growth characteristics were dependent on the type of EC, the nature of the substrates on which the ECs were grown and the presence of growth factors. For all types of ECs optimal growth and formation of a monolayer were observed when the ECs were cultured on fibronectin or gelatin substrates in the presence of EC growth factor and heparin. Under these conditions confluent cultures of macrovascular ECs reached maximal cell densities of 1,400-1,900 ECs/mm2, whereas microvascular ECs reached maximal cell densities of about 700-900 ECs/mm2. The cell cycle times calculated from the population-doubling time and the stathmokinetic index, respectively, amounted to 63 and 83 h for microvascular ECs, 33 and 35 h for venous macrovascular ECs, and 29 and 35 h for arterial macrovascular ECs.
在培养过程中，对来自脐带静脉和动脉的人大血管内皮细胞（ECs）以及来自包皮的微血管ECs的形态和生长特性进行了比较。通过延时显微电影摄影术和相差显微镜观察，发现不同类型的ECs在细胞形态和迁移活性方面存在差异。生长特性取决于ECs的类型、ECs生长的底物性质以及生长因子的存在。对于所有类型的ECs，当在纤连蛋白或明胶底物上，在EC生长因子和肝素存在的情况下进行培养时，观察到最佳生长和单层形成。在这些条件下，大血管ECs的汇合培养物达到的最大细胞密度为1400 - 1900个ECs/mm²，而微血管ECs达到的最大细胞密度约为700 - 900个ECs/mm²。分别根据群体倍增时间和静止动力学指数计算出的细胞周期时间，微血管ECs为63小时和83小时，静脉大血管ECs为33小时和35小时，动脉大血管ECs为29小时和35小时。
