Neuer G, Bautz F A, Bustin M, Michels H, Truckenbrodt H
Institute of Molecular Genetics, University of Heidelberg, Federal Republic of Germany.
Autoimmunity. 1994;17(1):23-30. doi: 10.3109/08916939409014655.
Autoantibodies against the nonhistone nucleosomal protein HMG-17 have been detected in a high percentage of ANA-positive patients with pauciarticular-onset JRA4. Here we report on the epitope mapping of the HMG-17 autoantigen with a set of overlapping and nested synthetic peptides spanning the entire amino acid sequence of the human HMG-17 protein. Competition ELISA experiments defined a proline and lysine rich octapeptide PKPEPKPK as the major epitope recognized by more than 70% of the HMG-17 positive JRA sera. Point mutations introduced in the autoimmune peptide determined the amino acid residues important for autoantibody recognition. Computer based sequence comparison shows close homology between the HMG-17 autoimmune epitope and certain infectious organisms, supporting the possibility that molecular mimicry is an important factor in the etiology of JRA.
在高比例的少关节起病型幼年类风湿关节炎（JRA）且抗核抗体（ANA）阳性的患者中，已检测到针对非组蛋白核小体蛋白HMG - 17的自身抗体。在此，我们报告了HMG - 17自身抗原的表位定位，所用材料为一组覆盖人HMG - 17蛋白整个氨基酸序列的重叠且嵌套的合成肽。竞争酶联免疫吸附测定（ELISA）实验确定了富含脯氨酸和赖氨酸的八肽PKPEPKPK为主要表位，超过70%的HMG - 17阳性JRA血清可识别该表位。在自身免疫肽中引入的点突变确定了对自身抗体识别重要的氨基酸残基。基于计算机的序列比较显示，HMG - 17自身免疫表位与某些感染性生物体之间具有高度同源性，这支持了分子模拟是JRA病因学中一个重要因素的可能性。
