Vlachoyiannopoulos P G, Boumba V A, Tzioufas A G, Seferiadis C, Tsolas O, Moutsopoulos H M
Department of Internal Medicine, School of Medicine, University of Ioannina, Greece.
J Autoimmun. 1994 Apr;7(2):193-201. doi: 10.1006/jaut.1994.1015.
Autoantibodies to HMG-17, a non-histone nucleosomal protein, were found in systemic lupus erythematosus (SLE), mixed connective tissue disease (MCTD) and ANA positive pauciarticular juvenile rheumatoid arthritis (JRA), but not in rheumatoid arthritis (RA). Using highly purified HMG-17 derived from porcine thymus, we tested sera from 50 patients with scleroderma for antibodies to HMG-17 by enzyme-linked immunosorbent assay (ELISA). There were 16 patients with diffuse cutaneous systemic sclerosis (dSSc) and 34 with limited cutaneous systemic sclerosis (1SSc) with age at disease onset 40.25 +/- 11.68 and 39.94 +/- 15.68 years, respectively, and disease duration 6.03 +/- 4.98 and 13.34 +/- 11.80 years, respectively (P < 0.0001). Anticentromere antibodies (ACA) were found in 65% of 1SSc patients but not in dSSc (P < 0.0001) while the prevalence of antinuclear antibodies (ANA) with other than ACA patterns did not differ in the two groups. Anti-HMG-17 antibodies occurred in 20 patients (40%), five with dSSc (31%) and 15 with 1SSc (41%). Twelve of the 20 HMG-17 positive patients were also positive for ACA (60%) but this association was not significant. No association was found between anti-HMG-17 and other antibody patterns. In conclusion, anti-HMG-17 antibodies occur in one third of scleroderma patients, do not discriminate scleroderma variants and are not associated with other autoantibodies.
在系统性红斑狼疮（SLE）、混合性结缔组织病（MCTD）及抗核抗体（ANA）阳性的少关节型幼年类风湿性关节炎（JRA）患者中发现了针对非组蛋白核小体蛋白HMG - 17的自身抗体，但在类风湿性关节炎（RA）患者中未发现。我们使用从猪胸腺中高度纯化的HMG - 17，通过酶联免疫吸附测定（ELISA）检测了50例硬皮病患者血清中针对HMG - 17的抗体。其中有16例弥漫性皮肤系统性硬化症（dSSc）患者和34例局限性皮肤系统性硬化症（lSSc）患者，发病年龄分别为40.25±11.68岁和39.94±15.68岁，病程分别为6.03±4.98年和13.34±11.80年（P<0.0001）。65%的lSSc患者检测到抗着丝点抗体（ACA），而dSSc患者中未检测到（P<0.0001），两组中具有非ACA模式的抗核抗体（ANA）患病率无差异。20例患者（40%）出现抗HMG - 17抗体阳性，其中5例dSSc患者（31%）和15例lSSc患者（41%）。20例HMG - 17阳性患者中有12例ACA也呈阳性（60%），但这种关联无统计学意义。未发现抗HMG - 17与其他抗体模式之间存在关联。总之，抗HMG - 17抗体在三分之一的硬皮病患者中出现，不能区分硬皮病的亚型，且与其他自身抗体无关。
