d'Angeac A D, Monier S, Pilling D, Travaglio-Encinoza A, Rème T, Salmon M
Unité INSERM 291, Montpellier, France.
Eur J Immunol. 1994 Jul;24(7):1503-11. doi: 10.1002/eji.1830240707.
CD3+ T cells expressing the 110-kDa CD57 antigen are found in survivors of renal, cardiac and bone marrow transplants, in patients with acquired immune deficiency syndrome and in patients with rheumatoid arthritis. They are also present in normal individuals and expand upon ageing. They do not grow in culture and their role in the immune response is poorly understood. The expression of the various isoforms of the leukocyte common antigen (CD45) identifies a spectrum of differentiation in CD4+ and CD8+ T cells ranging from naive (CD45RA+CD45RBbrightCD45RO-) through early primed cells (CD45RA-RBbrightROdull) to highly differentiated memory cells which are CD45RA-RBdullRObright. CD45 isoforms expressed by CD57+ T cells showed distinct differences between CD4+ and CD8+ populations, but in each case indicated an advanced state of differentiation. The expression of T cell receptor V beta families was highly variable between individuals, but both CD57+ and CD57- cells show a full range of the specificities tested. V beta expression was more closely related within either the CD4+ or the CD8+ subsets, irrespective of CD57 expression, than between these subsets, suggesting a relationship between CD57+ and CD57- cells within the same T cell pool. This possibility was supported by experiments showing that CD3+CD57+ lymphocytes were similar to CD3+CD57- T cells in terms of the production of basic T cell cytokines [interleukin (IL)-2, IL-4, and interferon-gamma]. Furthermore, in vitro stimulation of CD3+CD57- T cells in secondary mixed leukocyte reaction or by co-culture with IL-2 and IL-4 induced the appearance of CD3+CD57+ cells with phenotypic and functional similarities to in vivo CD3+CD57+ cells. These data strongly suggest that the expression of CD57 is a differentiation event which occurs on CD57- T cells late in the immune response.
表达110 kDa CD57抗原的CD3⁺ T细胞见于肾移植、心脏移植和骨髓移植的幸存者、获得性免疫缺陷综合征患者以及类风湿关节炎患者。它们也存在于正常个体中，并随年龄增长而增多。它们在培养中不生长，其在免疫反应中的作用尚不清楚。白细胞共同抗原（CD45）各种同工型的表达确定了CD4⁺和CD8⁺ T细胞的一系列分化阶段，从幼稚细胞（CD45RA⁺CD45RBbrightCD45RO⁻）到早期致敏细胞（CD45RA⁻RBbrightROdull），再到高度分化的记忆细胞（CD45RA⁻RBdullRObright）。CD57⁺ T细胞表达的CD45同工型在CD4⁺和CD8⁺群体之间显示出明显差异，但在每种情况下都表明处于分化的晚期状态。T细胞受体Vβ家族的表达在个体之间高度可变，但CD57⁺和CD57⁻细胞均显示出所检测的全部特异性。无论CD57表达如何，Vβ表达在CD4⁺或CD8⁺亚群内比在这些亚群之间更密切相关，这表明在同一T细胞库中CD57⁺和CD57⁻细胞之间存在关系。实验表明，就基本T细胞细胞因子[白细胞介素（IL）-2、IL-4和干扰素-γ]的产生而言，CD3⁺CD57⁺淋巴细胞与CD3⁺CD57⁻ T细胞相似，这支持了这种可能性。此外，在二次混合淋巴细胞反应中或通过与IL-2和IL-4共培养对CD3⁺CD57⁻ T细胞进行体外刺激，诱导出现了与体内CD3⁺CD57⁺细胞具有表型和功能相似性的CD3⁺CD57⁺细胞。这些数据强烈表明，CD57的表达是免疫反应后期发生在CD57⁻ T细胞上的分化事件。
