Morley J K, Batliwalla F M, Hingorani R, Gregersen P K
Department of Medicine, North Shore University Hospital, Manhasset, NY 11030, USA.
J Immunol. 1995 Jun 1;154(11):6182-90.
A number of recent reports have established that oligoclonality and/or clonal expansion is a common feature of the CD8+ T cell population. Oligoclonal expansion has also been observed in bone marrow transplant recipients and rheumatoid arthritis patients, disease states in which CD57+CD8+ T cells are occasionally elevated. In this study we have compared the TCR repertoire of the CD57+ and CD57- subsets of CD8+ T cells in normal persons by using three-color FACS analysis with a panel of 16 mAbs specific for TCR V segments. The CD57 surface marker was highly variable in frequency but generally present on a minority of CD8+CD3+ T cells (mean 16.3%, SD 12.7) in a group of 41 normal volunteers. Dramatic oligoclonal expansion was present in the CD57+CD8+ T cell population in 15 of 41 (37%) of our study population and thus is a characteristic feature of the normal immune system. No such prominent oligoclonal expansions were observed in the CD57-CD8+ subset, although preliminary experiments suggest that oligoclonality per se is occasionally present at a lower frequency in CD57- cells. The reasons for this persistent accumulation of oligoclonal CD8+CD57+ T cells and their function in immune homeostasis are unclear.
近期的一些报告证实，寡克隆性和/或克隆性扩增是CD8+ T细胞群体的一个常见特征。在骨髓移植受者和类风湿性关节炎患者中也观察到了寡克隆性扩增，在这些疾病状态下，CD57+CD8+ T细胞偶尔会升高。在本研究中，我们通过使用一组针对TCR V区段的16种单克隆抗体进行三色荧光激活细胞分选分析，比较了正常人CD8+ T细胞中CD57+和CD57-亚群的TCR库。在一组41名正常志愿者中，CD57表面标志物的频率变化很大，但通常存在于少数CD8+CD3+ T细胞上（平均16.3%，标准差12.7%）。在我们研究的41名受试者中有15名（37%）的CD57+CD8+ T细胞群体中存在显著的寡克隆性扩增，因此这是正常免疫系统的一个特征。在CD57-CD8+亚群中未观察到如此明显的寡克隆性扩增，尽管初步实验表明寡克隆性本身在CD57-细胞中偶尔也会以较低频率出现。寡克隆性CD8+CD57+ T细胞持续积累的原因及其在免疫稳态中的功能尚不清楚。
