Seyschab H, Bretzel G, Friedl R, Schindler D, Sun Y, Hoehn H
Department of Human Genetics, University of Würzburg, Germany.
Mutat Res. 1994 Jul 16;308(2):149-57. doi: 10.1016/0027-5107(94)90150-3.
The effect of caffeine on the endogenous G2 phase cell cycle blockage of Fanconi anemia (FA) cells was compared with the effect of caffeine on the G2 phase blockage induced in control cells by X-irradiation. The G2 phase accumulations in FA cells could be completely resolved by exposure to 1.5 mM caffeine. This was also observed in three brothers with endogenous G2 phase blockage due to unusual BrdU sensitivity. In contrast, G2 phase blockage induced by X-irradiation was only partially resolved by exposure to caffeine. The rescued G2 phase cells from FA patients were arrested within the following G1 phase compartments. This was not seen in X-irradiated cells from control donors. These results point towards a different nature and/or repair mechanism of the endogenous G2 phase lesion in FA cells compared to that induced by X-irradiation in control cells.
将咖啡因对范可尼贫血（FA）细胞内源性G2期细胞周期阻滞的影响与咖啡因对X射线照射诱导的对照细胞G2期阻滞的影响进行了比较。通过暴露于1.5 mM咖啡因，FA细胞中的G2期积累可完全消除。在三个因异常的BrdU敏感性而内源性G2期阻滞的兄弟中也观察到了这一点。相比之下，X射线照射诱导的G2期阻滞仅通过暴露于咖啡因得到部分缓解。从FA患者中挽救的G2期细胞在随后的G1期隔室中停滞。在对照供体的X射线照射细胞中未观察到这种情况。这些结果表明，与对照细胞中X射线照射诱导的损伤相比，FA细胞内源性G2期损伤具有不同的性质和/或修复机制。
