Hirose S, Nakanishi K, Yoshimoto T, Kashiwamura S, Hiroishi K, Matsumoto H, Hamaoka T, Shinka S, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
J Immunol. 1994 Aug 15;153(4):1847-57.
The responsiveness of CD5- and CD5+ B cells of BALB/c and NZB/WF1 mice to various cytokines was examined with respect to their growth and differentiation. BALB/c splenic CD5- B cells required longer incubation with IL-2 or pretreatment with IL-4 to respond to IL-2 by DNA synthesis, whereas NZB/WF1 CD5- B cells were highly competent to IL-2. Flow cytometric analysis demonstrated that NZB/WF1 and BALB/c CD5- B cells had higher and intermediate proportions of B cells positive for IL-2R beta, respectively. On the other hand, BALB/c and NZB/WF1 splenic CD5+ B cells consisted of lower proportion of B cells positive for IL-2R beta than did their corresponding CD5- B cells and grew meagerly in response to IL-2. Peritoneal exudative CD5+ B cells of NZB/WF1 mice lacked IL-2R beta mRNA expression and failed to respond to IL-2. Although both BALB/c and NZB/WF1 CD5- B cells pretreated with anti-IgM, IL-4, and IL-5 responded to IL-2 by DNA synthesis, only BALB/c CD5- B cells developed into IgM-producing cells. Furthermore, BALB/c, but not NZB/WF1 CD5-, B cells pretreated with anti-IgM and IL-5 responded to IL-2 by IgM production without DNA synthesis. Thus, cross-talk between IL-4 and IL-2 operated in the growth responses of both BALB/c and NZB/WF1 splenic CD5- B cells, whereas cross-talk between IL-5 and IL-2 operated only in the differentiation of BALB/c CD5- B cells, providing us with another intriguing functional abnormality of NZB/WF1 B cells.
研究了BALB/c和NZB/WF1小鼠的CD5⁻和CD5⁺ B细胞对各种细胞因子的反应，涉及它们的生长和分化。BALB/c脾CD5⁻ B细胞需要更长时间与IL-2孵育或用IL-4预处理才能通过DNA合成对IL-2作出反应，而NZB/WF1 CD5⁻ B细胞对IL-2具有高度反应性。流式细胞术分析表明，NZB/WF1和BALB/c CD5⁻ B细胞中IL-2Rβ阳性B细胞的比例分别较高和中等。另一方面，BALB/c和NZB/WF1脾CD5⁺ B细胞中IL-2Rβ阳性B细胞的比例低于相应的CD5⁻ B细胞，并且对IL-2的反应生长微弱。NZB/WF1小鼠的腹腔渗出CD5⁺ B细胞缺乏IL-2Rβ mRNA表达，对IL-2无反应。尽管用抗IgM、IL-4和IL-5预处理的BALB/c和NZB/WF1 CD5⁻ B细胞都通过DNA合成对IL-2作出反应，但只有BALB/c CD5⁻ B细胞发育为产生IgM的细胞。此外，用抗IgM和IL-5预处理的BALB/c而非NZB/WF1 CD5⁻ B细胞通过产生IgM对IL-2作出反应而无DNA合成。因此，IL-4和IL-2之间的相互作用在BALB/c和NZB/WF1脾CD5⁻ B细胞的生长反应中起作用，而IL-5和IL-2之间的相互作用仅在BALB/c CD5⁻ B细胞的分化中起作用，这为我们提供了NZB/WF1 B细胞的另一个有趣的功能异常。
